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FISH for Pre-implantation Genetic Diagnosis
JoVE 杂志
医学
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JoVE 杂志 医学
FISH for Pre-implantation Genetic Diagnosis

FISH for Pre-implantation Genetic Diagnosis

DOI:
10.3791/2570-v

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07:34 min

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February 23, 2011

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, ,
1Department of Cytogenetics, GSTS-Pathology, Guy’s & St Thomas’ NHS Foundation Trust,Guy’s & St Thomas’ Centre for Preimplantation Genetic Diagnosis

Chapters

  • 00:05Title
  • 01:16Lysing and Spreading a Nucleus from a Biopsied Blastomere
  • 02:38In Situ Hybridization of a Single Blastomere Nucleus
  • 05:50FISH Diagnosis
  • 07:08Conclusion

Summary

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This article describes the selection of suitable probes for single-cell FISH, spreading techniques for blastomere nuclei, and in situ hybridization and signal scoring, applied to pre-implantation genetic diagnosis (PGD) in a clinical setting.

Tags

Pre-implantation Genetic DiagnosisPGDAssisted Reproduction TechnologyARTMonogenic DiseaseCystic FibrosisChromosome RearrangementRecurrent MiscarriagesInfertilityOvarian StimulationIn Vitro FertilizationIVFIntracytoplasmic Sperm InjectionICSICleavage-stage EmbryosBiopsyFluorescence In Situ HybridizationFISHChromosome ImbalanceX-linked DiseaseMutation-specific TestChromosome AneuploidyPGS

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