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Kültür Hipokampal Nöronlar Dendritik Spine Floresan Takip edilen Proteinler Photobleaching sonra Floresan Kurtarma (sıkı bağlamak)
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
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Kültür Hipokampal Nöronlar Dendritik Spine Floresan Takip edilen Proteinler Photobleaching sonra Floresan Kurtarma (sıkı bağlamak)

53,521 Views

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06:35 min

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April 16, 2011

DOI:

10.3791/2568-v

DOI:
10.3791/2568-v

•

06:35 min
April 16, 2011

•

12 Views
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1National Institute on Deafness and Other Communication Disorders,National Institutes of Health, Bethesda

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Fluorescence Recovery After Photobleaching (FRAP)GFP-tagged ProteinsDendritic SpinesCultured Hippocampal NeuronsMobilityExcitation LaserFluorescence Recovery RateProtein Turnover RateEGFP VectorZeiss 710 Confocal MicroscopePhotobleachingTime Lapse ImagesMobile FractionImmobile FractionImageJ SoftwareGraphpad Software

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Zheng, C., Petralia, R. S., Wang, Y., Kachar, B. Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons. J. Vis. Exp. (50), e2568, doi:10.3791/2568 (2011).

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