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Fluorescence Recovery After Photobleaching (FRAP) der Fluoreszenz-markierten Proteine in dendritischen Dornen von hippokampalen Neuronen

JoVE 杂志
神经科学

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JoVE 杂志神经科学

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons

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Fluorescence Recovery After Photobleaching (FRAP) der Fluoreszenz-markierten Proteine in dendritischen Dornen von hippokampalen Neuronen

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06:35 min

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April 16, 2011

DOI:

DOI:

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06:35 min

April 16, 2011

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12 Views

Chan-Ying Zheng, Ronald S. Petralia, Ya-Xian Wang, Bechara Kachar

¹National Institute on Deafness and Other Communication Disorders,National Institutes of Health, Bethesda

Tags

Fluorescence Recovery After Photobleaching (FRAP)GFP-tagged Proteins Dendritic Spines Cultured Hippocampal Neurons Mobility Excitation Laser Fluorescence Recovery Rate Protein Turnover Rate EGFP Vector Zeiss 710 Confocal Microscope Photobleaching Time Lapse Images Mobile Fraction Immobile Fraction ImageJ Software Graphpad Software

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