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Organotipica Culture cerebellare: sfide apoptotiche e rilevamento
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May 17, 2011
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Questo metodo descrive la generazione di organotipica culture cerebellare e l'effetto di alcuni stimoli apoptotici sulla vitalità dei diversi tipi di cellule del cervelletto.
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Organotypic Cerebellar CulturesApoptotic ChallengesDetection神经科学Organotypic CulturesPreparationSlicesStaining ProcedureBrain PartsHippocampusCerebellumCellular OrganizationNeuronal NetworksPurkinje CellsDissociated Primary CultureDevelopmental FeaturesIn Vitro StudiesElectrophysiological ExperimentsPharmacological ExperimentsApoptotic StimuliFas LigandTUNEL StainingCell Death Measurement
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Hurtado de Mendoza, T., Balana, B., Slesinger, P. A., Verma, I. M. Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection. J. Vis. Exp. (51), e2564, doi:10.3791/2564 (2011).
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