This content is Free Access.
Expansão, Purificação e Avaliação Funcional de Células NK do sangue periférico
47,376 Views
•
10:44 min
•
February 02, 2011
DOI:
Summary
Automatically generated
Aqui nós descrevemos um método eficiente para expandir e purificar um grande número de células NK e avaliar a sua função.
Explore More Videos
ExpansionPurificationFunctional AssessmentHumanPeripheral BloodNK CellsNatural Killer CellsImmune SurveillanceInfectious MicroorganismsTumorsLimited AvailabilityIn VitroNK Cell ImmunotherapyMethodEfficiently ExpandVast QuantitiesFunctional NK CellsEx VivoK562 Cells Expressing Membrane-bound IL21Artificial Antigen-presenting Cell (aAPC)Cell ProductSteady-state LeukapheresisDonorDepletion Of T CellsPositive Selection Of NK CellsIL-2 Overnight ActivationSmall Numbers Of NK Cells Delivered In Clinical TrialsInability To Propagate NK Cells In VitroGenerating Sufficient Cell Numbers
Read Article
Cite this Article
Somanchi, S. S., Senyukov, V. V., Denman, C. J., Lee, D. A. Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells. J. Vis. Exp. (48), e2540, doi:10.3791/2540 (2011).
Copy