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神经科学
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Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

DOI:
10.3791/2439-v

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10:06 min

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February 15, 2011

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, , ,
1Swedish Medical Nanoscience Center, Department of Neuroscience,Karolinska Institutet

Chapters

  • 00:05Title
  • 02:13Preparing for Slicing and Culturing
  • 03:17SCN Slicing Procedure
  • 06:06Organotypic SCN Culture
  • 08:09Results: Circadian Bioluminescence in SCN Slices
  • 09:27Conclusion

Summary

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The procedure of preparing slices containing the adult mouse hypothalamic suprachiasmatic nucleus (SCN), and a rapid way to culture the SCN tissue in organotypic culture condition, are reported. Further, the measurement of oscillatory clock gene protein expression using dynamic luciferase reporter technology is described.

Tags

Slice PreparationLuciferase RecordingClock Gene ActivitySuprachiasmatic NucleusCircadian ClockPhysiology行为学RetinaOptic NerveNegative Feedback LoopsClock GenesPeriod GenesCore ClockMembrane DepolarizationCalciumCAMPOscillationsGene ExpressionMetabolic ActivityElectrical ActivityIn Vitro StudyMolecular InvestigationsElectrophysiological InvestigationsMetabolic Investigations

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