Journal
/
神经科学
/
Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
JoVE 杂志
神经科学
Author Produced
This content is Free Access.
JoVE 杂志 神经科学
Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

13,333 Views

•

09:13 min

•

November 11, 2010

DOI:

10.3791/2384-v

DOI:
10.3791/2384-v

•

09:13 min
November 11, 2010

•

3 Views
, , , ,
1Department of Neurobiology and Behavior,University of California, Irvine

Explore More Videos

CryopreservationNeuronal CulturesFreezing MediumDimethyl Sulfoxide (DMSO)Hank’s Buffered Salt Solution (HBSS)CryovialsRate-controlled Freezing ContainerPost-thaw ProcessingDissociationNeuronal-enriched CulturesNeuritic GrowthImmunocytochemical StainingAstrocytic MarkerGlial Fibrillary Acidic Protein (GFAP)Neuronal MarkerBeta-tubulin Class IIINeural Precursor Cell CulturesNeurospheresDifferentiating Conditions

Read Article

Cite this Article

Rahman, A. S., Parvinjah, S., Hanna, M. A., Helguera, P. R., Busciglio, J. Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures. J. Vis. Exp. (45), e2384, doi:10.3791/2384 (2010).

Download .ris file

Copy

Share Video

.

Copy