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神经科学
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Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

DOI:
10.3791/2330-v

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14:57 min

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March 23, 2011

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, ,
1Graduate Center for Gerontology,University of Kentucky College of Public Health, 2Department of Molecular and Biomedical Pharmacology,University of Kentucky College of Medicine, 3Sanders-Brown Center on Aging,University of Kentucky College of Medicine

Chapters

  • 00:05Title
  • 01:52Brain Removal and Hippocampal Dissection in Aged Rats
  • 06:41Brain Sectioning Using A Vibrating Microtome
  • 08:12Elicit and Record CA3-CA1 Synaptic Responses
  • 11:32Representative Results
  • 14:31Conclusion

Summary

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This article outlines procedures for preparing hippocampal slices from rats and transgenic mice for the study of synaptic alterations associated with brain aging and age-related neurodegenerative diseases, such as Alzheimer’s disease.

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Acute Hippocampal SlicesRatsTransgenic MiceSynaptic AlterationsAgingAmyloid PathologySynaptic FunctionPlasticityElectrophysiological TechniquesCognitive ImpairmentsSynaptic Plasticity MechanismsLong-term PotentiationLong-term DepressionNootropic CompoundsBrain AgingAlzheimer’s Disease (AD)Aged RatsAD Model Mice

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