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Cryopreserving和恢复人类iPS细胞血清替代馈线中使用完整的基因敲除
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06:47 min
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July 15, 2010
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这一协议描述为cryopreserving淘汰赛SR冻存介质的人类iPS细胞，这些细胞恢复完整的基因敲除SR直属免费（KSR - FF）介质或进纸器为基础的基因敲除的SR介质的详细过程。
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CryopreservingRecoveringHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-free MediumReprogrammingFibroblastsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementEmbryonic Stem Cell GrowthIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Or Human Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated Cells
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Wagner, K., Welch, D. Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium. J. Vis. Exp. (41), e2237, doi:10.3791/2237 (2010).
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