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Isolation of Retinal Stem Cells from the Mouse Eye
JoVE 杂志
生物学
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JoVE 杂志 生物学
Isolation of Retinal Stem Cells from the Mouse Eye

Isolation of Retinal Stem Cells from the Mouse Eye

DOI:
10.3791/2209-v

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07:22 min

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September 11, 2010

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,
1Molecular Genetics,University of Toronto

Chapters

  • 00:00Title
  • 01:05Introduction
  • 01:21Isolating Retinal Stem Cells from the Mouse Eye
  • 06:27Results of Isolating Retinal Stem Cells
  • 07:02Conclusion

Summary

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In this video, we will demonstrate how to isolate retinal stem cells from the ciliary epithelium of the mouse eye and grow them in culture to form clonal retinal spheres. The spheres that are isolated possess the cardinal properties of stem cells: self-renewal and multipotentiality.

Tags

IsolationRetinal Stem CellsMouse EyeCiliary EpitheliumAdult MouseQuiescent CellCEPigmented CellNon-pigmented CellNeural RetinaRPEControversySpheresDifferentiationRegenerationInjuryMammalian EyeStem Cell IdentificationMechanismsQuiescenceTransplantationCiliary Epithelial Cells

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