需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
35,185 Views
•
11:30 min
•
December 18, 2010
DOI:
Summary
Automatically generated
The colony forming cell (CFC) assay is an in vitro assay in which hematopoietic progenitors form colonies in a semi-solid medium. A combination of colony morphology, cell morphology, and flow cytometry are used to assess the ability of the progenitors to proliferate and differentiate along the different hematopoietic lineages.
Explore More Videos
Colony Forming Cell (CFC) AssayHuman Hematopoietic CellsBone MarrowCord BloodPeripheral BloodHematopoiesisLeukemogenesisLymphoid LineageMyeloid LineageSemisolid MediumProliferationDifferentiation PatternColoniesInput CellsFlow CytometryGiemsa-stained SlidesMyeloid DifferentiationOncogenesPrimary Human CD34+ CellsRetroviral ConstructNUP98-HOXA9MSCV-IRES-GFP
Read Article
Cite this Article
Sarma, N. J., Takeda, A., Yaseen, N. R. Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells. J. Vis. Exp. (46), e2195, doi:10.3791/2195 (2010).
Copy