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Upright Imaging of Drosophila Embryos
JoVE 杂志
生物学
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JoVE 杂志 生物学
Upright Imaging of Drosophila Embryos

Upright Imaging of Drosophila Embryos

DOI:
10.3791/2175-v

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07:34 min

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September 13, 2010

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, , , , , , ,
1Department of Biology,Case Western Reserve University, 2Department of Genetics,Case Western Reserve University

Chapters

  • 00:00Title
  • 00:53Introduction
  • 02:34Mounting Drosophila Embryos in Glycerin Jelly for Imaging
  • 04:28Cutting the Embryos
  • 05:25Visualization and Imaging of Embryos
  • 06:17Representative Images
  • 06:40Conclusion

Summary

自动翻译

自动翻译

Here we present a mounting protocol for stained Drosophila embryos in an upright position that allows imaging of cross-sections using Confocal microscopy.

Tags

Upright ImagingDrosophila EmbryosMorphogenetic GradientsCellular MovementsDorsal/ventral AxisCurrent TechniquesNew TechniqueMountingFixed EmbryosLabeled EmbryosCoronal ViewingConfocal ImagingGlycerin JellyEmbedding EmbryosJelly StripsUpright PositioningSandwiching EmbryosThin BeddingSlideAlignmentVisualizing EmbryosMicroscope StandPrecise MeasurementFluorescent Signals

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