需要订阅 JoVE 才能查看此.  登录或开始免费试用。
A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging

A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging

成績單

For popliteal draining lymph node harvest, apply mineral oil to the calf and knee on the injected side of the animal. Next, make a midline incision in the calf from the heel to the knee, and dissociate the calf musculature from the skin.

Expose the popliteal fossa. The popliteal lymph node will appear as a translucent sphere within the fossa. Then, remove the lymph node with the microsurgery curved forceps.

Related Videos

Read Article