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RNAi筛选参与宿主因素牛痘病毒感染使用果蝇细胞
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells
DOI:

08:20 min

August 25, 2010

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Chapters

  • 00:00Title
  • 00:58Introduction
  • 01:19RNA Interference in 384-well Plates
  • 03:09Infecting with Vaccinia Virus
  • 04:11Staining Plates
  • 06:02Representative Images and Interpretation of Infection Rates
  • 07:35Conclusion

Summary

自动翻译

在病毒感染的新型主机所涉及的因素可以识别通过细胞为基础的的全基因组功能RNAi筛选的损失。一<em>果蝇</em>细胞培养模型的易用性和效率的RNAi由于这种方法特别适合。在这里,我们展示了这种技术使用<em>牛痘</em>病毒为例。

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