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Oral Administration of Rotenone using a Gavage and Image Analysis of Alpha-synuclein Inclusions in the Enteric Nervous System
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Oral Administration of Rotenone using a Gavage and Image Analysis of Alpha-synuclein Inclusions in the Enteric Nervous System

Oral Administration of Rotenone using a Gavage and Image Analysis of Alpha-synuclein Inclusions in the Enteric Nervous System

DOI:
10.3791/2123-v

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11:53 min

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October 26, 2010

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,
1Institute of Anatomy,Technische Universität Dresden

Chapters

  • 00:00Title
  • 01:00Introduction
  • 02:51Intragastric Adminstration of Rotenone
  • 04:00Immunostaining Procedures
  • 04:41Alpha-synuclein Inclusion Image Analysis
  • 07:58Representative Results
  • 10:39Conclusion

Summary

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自动翻译

Parkinson's disease has been related to the exposure to pesticides. Here we show a method to deliver pesticides using a gastric tube at the desired concentration and a method to analyze their effect in alpha-synuclein accumulation in the enteric nervous system.

Tags

Rotenone AdministrationGavage MethodImage AnalysisAlpha-synuclein InclusionsEnteric Nervous SystemParkinson’s Disease PathologyNeuropathological StagingOlfactory BulbDorsal Motor Nucleus Of The VagusIntermediolateral Nucleus Of The Spinal CordSubstantia NigraPesticide ExposureRotenone Effects On ENSMitochondrial Complex I InhibitionLipophylic PesticideGastrointestinal AbsorptionHepatoportal SystemPesticide Administration Method

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