Journal
/
免疫与感染
/
Generation of Recombinant Influenza Virus from Plasmid DNA
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Generation of Recombinant Influenza Virus from Plasmid DNA

Generation of Recombinant Influenza Virus from Plasmid DNA

DOI:
10.3791/2057-v

•

11:31 min

•

August 03, 2010

•

,
1Department of Microbiology and Immunology,University of Rochester School of Medicine and Dentistry, 2Departments of Microbiology and Medicine, and Global Health and Emerging Pathogens Institute,Mount Sinai School of Medicine

Chapters

  • 00:04Title
  • 01:00Introduction
  • 01:29Influenza Virus Rescue Transfection
  • 04:54Infecting MDCK Cells & TC Harvest
  • 06:14Egg Infection & Allonotic Fluid Harvest
  • 07:55HA Assay
  • 08:55Confirmation of Rescue
  • 10:19Conclusion

Summary

自动翻译

自动翻译

Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.

Tags

Recombinant Influenza VirusPlasmid DNAReverse GeneticsInfluenza ResearchInfectious VirusesViral ProteinsMutationsViral ReplicationPathogenesisViral VectorsForeign Proteins

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

High-throughput Detection Method for Influenza Virus

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells

Rescue of Recombinant Newcastle Disease Virus from cDNA

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

Influenza Virus Propagation in Embryonated Chicken Eggs

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Read Article