This content is Free Access.
1Department of Pathology, Hershey Medical Center,Pennsylvania State University, 2Department of Chemistry,Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center,Pennsylvania State University, 4Materials Research Institute,Pennsylvania State University
Chapters
- 00:04Title
- 00:12Introduction
- 01:39Preparation of PF Library-DNA Fragments
- 03:26Preparation of S100B-bound Beads
- 04:55Selection & Recovery of Aptamers
- 06:40TOPO Cloning and Sequencing
- 07:17Sandwich Binding Assays
- 09:11Representative Results
- 10:35Conclusion
Protocolli di SELEX comprendono vari cicli di selezione, ognuno dei quali richiedono la rigenerazione di leganti legato, che a loro volta richiedono sequenze primer fisso fiancheggianti le regioni biblioteca casuale. Queste sequenze di innesco fisso può interferire con il processo di selezione (falsi positivi e negativi). Qui vi presentiamo un primer senza protocollo.
Tags
Primer-Free Aptamer SelectionRandom DNA LibraryAptamersOligonucleotidesDNARNAAffinitiesAntibodiesIn Vitro Selection ProcessSystematic Evolution Of Ligands By EXponential Enrichment (SELEX)TargetsSmall MoleculesProteinsMacromoleculesIn Vivo Diagnostic ApplicationsTherapeutic ApplicationsSpecificitySynthesizedProcessing ConditionsImmunogenicitySizeNucleotides (nt)Nucleic Acid LibrariesRandomized RegionsPrimer SitesSelection Process ComplicationsBioinformatics Approaches
