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Simplified and Efficient AAV Production Using HEK293 Suspension Culture
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HEK293 Suspension Cell Cultivation and Viral Vector Harvesting for Virus Production
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神经科学
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JoVE 杂志 神经科学
HEK293 Suspension Cell Cultivation and Viral Vector Harvesting for Virus Production

HEK293 Suspension Cell Cultivation and Viral Vector Harvesting for Virus Production

Simplified and Efficient AAV Production Using HEK293 Suspension Culture

DOI:
10.3791/201530-v

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03:26 min

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April 26, 2024

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, , , , , ,
1Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience,Royal Netherlands Academy of Arts and Sciences (KNAW), 2Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

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HEK293 CellsSuspension Cell CultivationViral Vector HarvestingVirus ProductionCell CultureTransfectionCell LysisPEG PrecipitationViral Vector Purification

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