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One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
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June 25, 2010
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The urease method of sample preparation for GC/MS analysis of intermediary metabolites is presented by its inventor. The method allows one-step follow-up of newborn screening for inborn errors by tandem mass spectrometry by quantifying carbohydrates, organic and amino acids all in a single process.
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One-step MetabolomicsCarbohydratesOrganic AcidsAmino AcidsTandem Mass SpectrometryAcylcarnitinesOrganic AcidemiasAmino Acid QuantificationEnzymatic TestingCarbohydrate DisordersUrease MethodSample PreparationGas ChromatographyDehydrationDerivatizationTrimethylsilylationCatalystTriethylammonium TrifluoroacetateAutomated InjectionChromatographyQuantification Of MetabolitesSemi-quantificationMass SpectraTMS Derivatized Biological Compounds
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Shoemaker, J. D. One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure. J. Vis. Exp. (40), e2014, doi:10.3791/2014 (2010).
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