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Mouse Brain Histone Modification Quantification Using Flow Cytometry
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Mouse Brain Immune-Enriched Fragment Isolation for Histone PTM Quantification
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Mouse Brain Immune-Enriched Fragment Isolation for Histone PTM Quantification

Mouse Brain Immune-Enriched Fragment Isolation for Histone PTM Quantification

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01:39 min

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September 15, 2023

DOI:

10.3791/200980-v

DOI:
10.3791/200980-v

•

01:39 min
September 15, 2023

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1 Views
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1Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, 2Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

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Mouse BrainImmune-enriched FragmentHistone PTM QuantificationIsotonic Density GradientMurine Brain HomogenateFax Buffer37% Density Gradient70% Density GradientCentrifugationMyelinImmune-enriched FragmentFax WashCell Pellet

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Mouse Brain Immune-Enriched Fragment Isolation for Histone PTM Quantification. J. Vis. Exp. (Pending Publication), e200980, doi:10.3791/200980 (2023).

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