/

/

EsiRNA MISSION pour le dépistage de l'ARNi dans les cellules de mammifères

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

MISSION esiRNA for RNAi Screening in Mammalian Cells

Please note that all translations are automatically generated. Click here for the English version.

EsiRNA MISSION pour le dépistage de l'ARNi dans les cellules de mammifères

15,875 Views

•

15:31 min

•

May 12, 2010

DOI:

DOI:

•

15:31 min

May 12, 2010

•

3 Views

Mirko Theis, Frank Buchholz

¹Max Planck Institute of Molecular Cell Biology and Genetics

Tags

MISSION EsiRNA RNAi Screening Mammalian Cells Gene Expression Control Small Interfering RNAs (siRNAs)Dicer RNA-induced Silencing Complex (RISC)Target MRNA Cleavage Loss Of Gene Function Studies Chemical Synthesis Of SiRNAs Endonucleolytic Cleavage Target-specific Long Double-stranded RNAs RNase III Endoribonuclease-prepared SiRNA Efficacy Of SiRNAs And EsiRNAs Off-target Effects

Read Article