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Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

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Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion

JoVE 杂志
生物化学

This content is Free Access.

JoVE 杂志生物化学

Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion

Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion

Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

DOI:

10.3791/200737-v

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02:21 min

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August 25, 2023

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Sarah Wazir, Danny Farhat, Mahalashmi Srinivasan², Jyh-Yeuan Lee

¹Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, ²Department of Biochemistry, Faculty of Medicine and Dentistry,University of Alberta

Tags

Crystallization Heterodimeric ABCG5/G8 Protein Lipidic Bicelle Hanging Drop Vapor Diffusion Bicelle Stock Solution DMPC CHAPSO Protein-bicelle Mixture Crystallization Reservoir Solution Crystal Growth Sodium Malate Cryo Loops Data Collection

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