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Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7
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Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection (Video) | JoVE
JoVE 杂志
生物学
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JoVE 杂志 生物学
Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection

Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection (Video) | JoVE

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

DOI:
10.3791/200471-v

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02:33 min

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June 02, 2023

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, , , ,
1Department of Pathology and Cell Biology,Columbia University Irving Medical Center, 2Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center,Columbia University Irving Medical Center

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Keywords: Yeast CellsMitochondriaHyPer7MicroscopyImagingFluorescenceConfocal MicroscopySCMOS CameraBinningLaser PowerExposureDynamic RangeNoise LevelTime-lapseZ-stacksTransmitted Light

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