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Crude Vector Production and Efficient DNA Delivery in Cell Cultures

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Production of Recombinant Adeno-Associated Viral Vectors and Transduction of Cultured Cells

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

Production of Recombinant Adeno-Associated Viral Vectors and Transduction of Cultured Cells

Production of Recombinant Adeno-Associated Viral Vectors and Transduction of Cultured Cells

Crude Vector Production and Efficient DNA Delivery in Cell Cultures

DOI:

10.3791/200291-v

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05:10 min

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October 20, 2023

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Brian Benyamini, Meagan N. Esbin², Oscar Whitney, Nike Walther, Anna C. Maurer

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²Biophysics Graduate Group,University of California, Berkeley

Tags

Recombinant Adeno-associated Viral Vectors Cell Transduction Plasmid Transfection Polyethylenimine Cell Culture Cell Lysis Virus Production Virus Purification

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