Journal
/
生物化学
/
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
/
Preparation of siRNA Mixture for Mitochondrial DNA Synthesis
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Preparation of siRNA Mixture for Mitochondrial DNA Synthesis

Preparation of siRNA Mixture for Mitochondrial DNA Synthesis

High-Throughput Quantification of the Synthesis and Distribution of mtDNA

DOI:
10.3791/200039-v

•

00:45 min

•

May 05, 2023

•

, , ,
1Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, 2Institute of Biochemistry and Biophysics,Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw

Tags

SiRNAMitochondrial DNASmall Interfering RNARNAiMAXTransfectionMicroplateCell CultureReagent Dispenser

Embed

添加到播放列表

Usage Statistics

Related Videos

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Preparation of siRNA Mixture for Mitochondrial DNA Synthesis

Cell Transfection and 5-Bromo-2'-Deoxyuridine (BrdU) Incorporation in HeLa Cells

5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells

HeLa Cell Fixation with Hoechst 33342 and Blocking

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells

Read Article