Journal
/
生物学
/
Cell Electrofusion Visualized with Fluorescence Microscopy
JoVE 杂志
生物学
Author Produced
This content is Free Access.
JoVE 杂志 生物学
Cell Electrofusion Visualized with Fluorescence Microscopy

Cell Electrofusion Visualized with Fluorescence Microscopy

DOI:
10.3791/1991-v

•

05:02 min

•

July 01, 2010

•

, ,
1Faculty of Electrical Engineering,University of Ljubljana

Chapters

  • 00:00Title
  • 00:20Loading the Cells with Cell Trackers CMFDA and CMRA
  • 02:31Electrofusion
  • 03:39Image Acquistion and Determination of the Fusion Yield

Summary

自动翻译

自动翻译

In this video we demonstrate efficient electrofusion of cells in vitro by means of modified adherence method using electroporation and the subsequent detection of fused cells visualization with fluorescence microscopy.

Tags

Cell ElectrofusionFluorescence MicroscopyHybrid CellsHuman TherapyNon-viral MethodNon-chemical MethodHigh-voltage Electric PulsesCell Plasma MembranesPermeabilityFusionLipid BilayersCytoplasmAdherence MethodCell Tracker DyesOrange CMRAGreen CMFDAFusion Yield

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Time-lapse Imaging of Mitosis After siRNA Transfection

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

Visualizing Stromule Frequency with Fluorescence Microscopy

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Read Article