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Lapso de tempo de imagens de Mitose Depois de Transfection siRNA
JoVE 杂志
生物学
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JoVE 杂志 生物学
Time-lapse Imaging of Mitosis After siRNA Transfection

Lapso de tempo de imagens de Mitose Depois de Transfection siRNA

16,627 Views

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08:21 min

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June 06, 2010

DOI:

10.3791/1878-v

DOI:
10.3791/1878-v

•

08:21 min
June 06, 2010

•

3 Views
, ,
1Department of Oncological Sciences, Huntsman Cancer Institute,University of Utah, 2Fluorescence Microscopy Core Facility,University of Utah

Summary

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Aqui nós descrevemos um protocolo básico para a imagem e quantificar o tempo de mitose de células vivas de tecidos de mamíferos cultura após transfecção siRNA.

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Time-lapse ImagingMitosisSiRNA TransfectionCellular OrganizationChromosome DynamicsFluorescence MicroscopyGFP-tagged ProteinsRNAi-based DepletionMitotic Regulatory ProteinAutomated Fluorescence MicroscopeImage SequencesStill-image MontagesMoviesQuantification Of Mitotic StagesMCherry-tagged Histone H2BTime-lapse Experiment Design

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Cite this Article

Mackay, D. R., Ullman, K. S., Rodesch, C. K. Time-lapse Imaging of Mitosis After siRNA Transfection. J. Vis. Exp. (40), e1878, doi:10.3791/1878 (2010).

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