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Chapters
- 00:00Title
- 00:14Introduction
- 02:10Preparation of Murine Splenocytes for CD40 – Stimulation
- 04:19Preparation of Feeder Cells
- 07:59CD40 – Stimulation
- 08:50Subcultivation
- 11:25Analysis of Murine CD40 – Activated B Cells
- 11:47Conclusion
Tags
CD40-activationB CellsAntigen PresentationInterleukin-4CD40 LigandExpansionPeripheral BloodCD40-B CellsLymph Node Homing MoleculesT CellsAntigensMemory T CellsImmuno-stimulatory Antigen-presenting Cells (APC)Cell-based ImmunotherapyMurine CD40-activated B CellsSplenocytesPurified B CellsC57BL/6 MiceHaplotype H-2bDensity Gradient CentrifugationHeLa CellsRecombinant Murine CD40 Ligand (tmuCD40L HeLa)
