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Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae
Chapters
- 00:00Title
- 00:36Introduction
- 01:12Sharpening Recording and Reference Electrodes
- 03:08Set Up of Odor Delivery and Recording Systems
- 04:46Preparation of the Drosophila Antenna for Recordings
- 07:00Recording – Drosophila melanogaster
- 08:58Preparation of Anopheles Maxillary Palps for Recordings
- 10:55Recording – Anopheles gambiae
- 12:04Representative Electrophysiological Data
- 12:55Conclusion
Electrophysiological responses of olfactory sensory neurons to odorants can be measured in insects using single sensillum recordings. In this video article we will demonstrate how to perform single sensillum recordings in the antennae of the vinegar fly (Drosophila melanogaster) and the maxillary palps of the malaria mosquito (Anopheles gambiae).
Tags
Single Sensillum RecordingsInsectsDrosophila MelanogasterAnopheles GambiaeSense Of SmellOlfactory Sensory NeuronsSensillaOdorantsSensillum LymphOdorant Receptor ProteinsBasal Firing RateAction PotentialsExtracellular RecordingsRecording ElectrodeReference ElectrodeSpike AmplitudeSpike Sorting Techniques
