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Chapters
- 00:00Title
- 01:00Introduction
- 01:47Preparation for Incorporating Acp1- GFP in the DMA
- 03:16Mating and Diploid Selection
- 03:50Sporulation and Germination
- 04:48Selection of Both Alleles
- 05:28Microscopy
- 06:02Representative Results/ Outcome
- 06:28Conclusion
GFP-fusion proteins are widely used to visualize organelles by confocal microscopy. However, screening for mutations that affect the morphology of organelles generally requires individual mutagenesis and is time consuming. Here, we demonstrate a method to simultaneously incorporate organelle-GFP markers in almost 5,000 non-essential genes in yeast.
