Glutathione S-transferases (GSTs) are detoxification enzymes involved in the metabolism of numerous chemotherapeutic drugs. Overexpression of GSTs is correlated with cancer chemotherapy resistance. One way to counter this phenotype is to use inhibitors. This protocol describes a method using a spectrophotometric assay to screen for potential GST inhibitors.
Glutathione S-transferases (GSTs) are metabolic enzymes responsible for the elimination of endogenous or exogenous electrophilic compounds by glutathione (GSH) conjugation. In addition, GSTs are regulators of mitogen-activated protein kinases (MAPKs) involved in apoptotic pathways. Overexpression of GSTs is correlated with decreased therapeutic efficacy among patients undergoing chemotherapy with electrophilic alkylating agents. Using GST inhibitors may be a potential solution to reverse this tendency and augment treatment potency. Achieving this goal requires the discovery of such compounds, with an accurate, quick, and easy enzyme assay. A spectrophotometric protocol using 1-chloro-2,4-dinitrobenzene (CDNB) as the substrate is the most employed method in the literature. However, already described GST inhibition experiments do not provide a protocol detailing each stage of an optimal inhibition assay, such as the measurement of the Michaelis-Menten constant (Km) for CDNB or indication of the employed enzyme concentration, crucial parameters to assess the inhibition potency of a tested compound. Hence, with this protocol, we describe each step of an optimized spectrophotometric GST enzyme assay, to screen libraries of potential inhibitors. We explain the calculation of both the half-maximal inhibitory concentration (IC50) and the constant of inhibition (Ki)—two characteristics used to measure the potency of an enzyme inhibitor. The method described can be implemented using a pool of GSTs extracted from cells or pure recombinant human GSTs, namely GST alpha 1 (GSTA1), GST mu 1 (GSTM1) or GST pi 1 (GSTP1). However, this protocol cannot be applied to GST theta 1 (GSTT1), as CDNB is not a substrate for this isoform. This method was used to test the inhibition potency of curcumin using GSTs from equine liver. Curcumin is a molecule exhibiting anti-cancer properties and showed affinity towards GST isoforms after in silico docking predictions. We demonstrated that curcumin is a potent competitive GST inhibitor, with an IC50 of 31.6 ± 3.6 µM and a Ki of 23.2 ± 3.2 µM. Curcumin has potential to be combined with electrophilic chemotherapy medication to improve its efficacy.
Cytosolic glutathione S-transferase enzymes (GSTs, EC 2.5.1.18) catalyze the conjugation of glutathione (GSH) into various electrophilic compounds, such as chemotherapeutic agents, to detoxify and eliminate them easily from the body1. Seven isoforms of cytosolic GST have been identified as alpha, mu, pi, sigma, omega, theta, and zeta. GSTs are mainly expressed in the liver, testes, lungs, and gastrointestinal tract2. The GST alpha 1 (GSTA1) isoform is highly expressed in hepatocytes. The body heterogeneously expresses other subtypes, including GST pi 1 (GSTP1) predominantly in the brain, heart, and lungs, and GST mu 1 (GSTM1) in the liver and testes3. Although there is high sequence homology between GST isoforms, each exhibits substrate specificity and is implicated in drug metabolism and cancer in different ways, according to its differential expression4,5.
Electrophilic compounds either enter the body exogenously or are produced endogenously. Pesticides, prostaglandins, carcinogens, and chemotherapeutic drugs are some of the potential substrates for glutathione conjugation reactions6. For example, any electron-deficient reactive compound formed within a cell is likely to become an electrophilic substrate. Alkylating agents such as chlorambucil or melphalan are eliminated as conjugates of GSH catalyzed by GSTs, and increased levels of these enzymes have been correlated with resistance to these compounds6,7.
Another important role of cytosolic GSTs is regulating the activity of mitogen-activated protein kinases (MAPK) such as MAPK8 (also known as c-Jun N-terminal kinase, or JNK1) and MAP3K5 (also known as apoptosis signal-regulating kinase 1, or ASK1)8. Some isoforms in their monomeric conformation will bind to these proteins and thus block the phosphorylation cascade. Under normal conditions, the GSTP1 isoform will sequester MAPK8 (the activator of the c-Jun protein). Combining the c-Jun with the c-Fos protein forms the activator protein 1 (AP-1) transcription factor, which is responsible for transcribing pro-apoptotic genes. In stressed cells, the complex formed by GSTP1 and MAPK8 dissociates, c-Jun is activated, and the genes leading to apoptosis start to be expressed9. Greater expression of this GST isoform might therefore block the pathway, leading to increased cell viability, more cellular proliferation, and lower cellular sensitivity to chemotherapy. Similar scenarios may occur with paralogs of GSTP1, for example, GSTM1, which interacts with MAP3K510.
The roles played by GSTs in drug metabolism and in the sequestration of MAPKs led to the hypothesis that a greater expression of GSTs might be a sign of a tumoral resistance mechanism to chemotherapeutic treatment6,11. For example, GSTP1 is overexpressed in numerous cancers and its presence has been correlated with a poor prognosis and an increased incidence of relapse8. Polymorphism in these genes has also shown differential drug exposure and survival rates for patients presenting various diseases, reinforcing the idea that these enzymes are crucial to mechanisms of drug resistance. For instance, individuals with the GSTM1 null genotype are associated with lower drug clearance and better survival12,13. There are several potential means of countering this overexpression, such as the use of GSH analogues, prodrugs that are activated by conjugation with GSTs, or direct GST inhibitors14,15.
All these methods are currently under investigation, and a few compounds have begun clinical trials for their potential usage among patients. However, to the best of our knowledge, there are no compounds in use as GST inhibitors in clinical settings15. Indeed, a lack of specificity for certain isoforms or the depletion of GSH in normal cells, which may lead to toxicities caused by the accumulation of reactive oxygen species (ROS) in organ systems, are just some of the drawbacks which reduce the potential of GST inhibitors14,15. The risk that these compounds might exert other pharmacodynamic effects on the body are also limiting their usage. Ethacrynic acid, for instance, is the most widely studied GST inhibitor in laboratory environments, but because it is primarily used as a strong diuretic, this property limits its use in combination with other drugs in clinical settings. Curcumin is another natural compound successfully screened as a GST inhibitor. This molecule is a polyphenol ether extracted from the Curcuma longa species of turmeric. It has shown promising results as a possible treatment option against cancer by inducing the apoptosis of various kind of tumor cell lines16,17. The compound can regulate diverse cellular pathways, such as the tyrosine kinase18 or the GST pathway. Studies with pure proteins have shown its inhibition potency on GSTA1, GSTM1 and GSTP119,20. However, conflicting results were observed in cancer cells, where greater intracellular GST activity was measured when cells were treated with curcumin21. Thus, it is important to investigate half-maximal inhibitory concentration (IC50) and the constant of inhibition (Ki) of any putative GST inhibitor using a clearly described protocol with proper controls before planning any further cellular experiments.
Screening and testing potential new GST inhibitors is therefore of significant clinical interest, and any new compound found must be safe and efficient for use in combination with electrophilic drugs. Focusing research on isoform-specific inhibitors might enable GST inhibition in tumor tissues exhibiting specific patterns of GST expression, thus allowing the development of an effective combination therapy. Finding inhibitors with differential modes of inhibition might also be of interest. For instance, a competitive inhibitor that uses GSH as a substrate can induce its depletion. This reduction of the GSH concentration in cells was shown to induce oxidative stress in neurons, leading to apoptosis. Another common mode of inhibition—uncompetitive inhibition—cannot be reversed even if the substrate is present in high concentrations.
The rate of enzymatic activity is represented by the Michaelis-Menten constant (Km) and the maximum velocity (Vmax), which can be determined by plotting a Michaelis-Menten graph, with the substrate concentration against the velocity of the reaction23. Km is the concentration of substrate required to occupy half the enzymatic active sites, meaning that a high Km represent less affinity. Vmax represents the maximum velocity of the reaction, reached when all the active sites are occupied by the substrate. Km is equal to half Vmax. There are three most common modes of inhibition: competitive, uncompetitive, and noncompetitive. In case of competitive inhibition, the inhibitor binds to the active site of the enzyme and competes with the substrate. Hence, Vmax does not change after addition of the inhibitor, but Km increases, as more substrate is needed to counter the inhibition. Uncompetitive inhibition occurs only when the substrate forms a complex with the enzyme. In this case, as the level of inhibition depends on the substrate and enzyme concentration, Vmax and Km decrease when the inhibitor is added to the reaction. The last mode of inhibition is non-competitive and is a mix of the two other inhibition patterns. The inhibitor can bind to the active site of the enzyme whether the enzyme is bound to its substrate or not. Here Vmax decreases after addition of the inhibitor, but Km does not change24.
A spectrophotometric assay that measured the GST activity was first developed by Habig et al. in 1974 using 1-chloro-2,4-dinitrobenzene (CDNB) as the substrate for the reaction22. Conjugation between GSH and CDNB forms GS-DNB, which exhibits a maximum light absorbance at the wavelength of 340 nm, recordable with a spectrophotometer. Most of the technique explained below is derived from Habig et al., including information on the best settings and important optimization points for an inhibitory assay. The technique can be applied to screening potential GST inhibitors, whether chosen by rational drug selection using computational predictions or by literature review. How to adapt the protocol to newly synthesized GST proteins or specific isoforms is also discussed. For instance, testing the inhibitory potency of GST isoforms exhibiting a clinically relevant polymorphism or of single-nucleotide polymorphisms (SNPs) could be potential uses for this protocol, targeting patient-specific GSTs.
This protocol provides a quick, feasible, and effective method for the screening of potential GST inhibitors in vitro before any other functional studies. The steps needed to evaluate the most commonly measured characteristics of an enzymatic inhibitor are described: the inhibitory concentration 50 (IC50) that is concentration of inhibitor required to decrease the enzymatic activity by half; and the constant of inhibition (Ki) that represents the equilibrium constant of the dissociation between the inhibitor and the enzyme, characteristic of the affinity between these two molecules. These two values are measured by non-linear regression and using equations specific for each mode of inhibition, respectively. We also demonstrate the assessment of this pattern of inhibition, using Michaelis-Menten plots to determine the change of Vmax and Km after addition of the inhibitor23,25,26.
1. Preparation of the GST enzyme solution
NOTE: The procedure for preparing the enzyme solution depends upon whether or not the units of enzymatic activity are known before the assay. One enzymatic unit is the amount of enzyme needed to synthesize 1 µmol of product per minute. Enzymatic activity is represented in unit/mL or µmol/min/mL and depends on the dilution of the enzymatic solution. Specific enzymatic activity is represented in unit/mg or µmol/min/mg and depends solely on the purity of the solution. Both these characteristics are determined below. If the enzymatic unit of an isolated GST isoform is unknown, it must be estimated to adjust the enzymatic concentrations for each reaction and provide reproducible results.
2. Measurement of the Michaelis–Menten constant of the GST isoform for CDNB
NOTE: The procedure is explained for a CDNB substrate but can be applied to any other substrate, such as GSH. Each concentration of CDNB needs its own blank, as the absorbance at 340 nm will increase according to the CDNB concentration.
3. Absorbance of the potential GST inhibitor
NOTE: This step is performed to investigate whether the potential GST inhibitor used in the reaction produces a metabolite that increases absorbance at the wavelength being measured. If it does, the amount of inhibitor used will have an impact on the results and a specific blank should be prepared for each concentration.
4. Inhibition assay of GST and IC50 assessment
5. Assessment of the Kiand the type of inhibition
Michaelis-Menten constant (Km) of CDNB with GST from equine liver
Curcumin is a safe natural compound even after ingestion at higher doses27 that showed anticancer properties16. The inhibitory potency of this molecule has been demonstrated on human recombinant GSTs19,20. By using the described protocol, we evaluated curcumin’s effect on GST activity using a pool of GST isoforms from equine liver. According to the supplier, the specific activity of this mix of proteins is 25 U/mg. A stock solution of 0.1 U/mL was prepared by dissolving the lyophilized powder in the appropriate amount of water. Ethacrynic acid was used in parallel as a positive control as this compound is the most widely used GST inhibitor in studies. Steps in setting up a GST activity assay and testing of inhibitors is outlined in Figure 1.
The Km has to be defined for each enzyme substrate reaction because using the substrate concentration equivalent to Km would ensure no bias regarding the determination of the mode of inhibition28. Six different concentrations of CDNB, ranging from 0.5 mM to 5 mM, were tested to measure this parameter, using a fixed concentration of 2.5 mM GSH.
The reaction’s final volume was 200 µL, in a 96-well plate. A specific blank was used for each experiment, using the same concentration of CDNB as the test well because spontaneous conjugation of CDNB and GSH could occur and might increase absorbance. A final enzyme concentration of 0.01 unit/mL was used for all the reactions, by adding 20 µL of the stock solution to the assay mix. Absorbance at 340 nm was recorded every minute for 10 minutes. The velocity (in mM/min) of the reaction was calculated using Equation 2. A Michaelis–Menten graph was plotted of the substrate concentration (mM) against the velocity (µM/min) (Figure 2), and the Km of the reaction was calculated. The experiment was repeated until at least three sets of data showed similar results. The Km of CDNB with GST from equine liver was measured as 0.26 ± 0.08 mM. A concentration of 0.2 mM of CDNB was used for each inhibitory assay using this batch of GSTs.
No spontaneous formation of a metabolite that absorbed at 340 nm was measured during experiments using curcumin incubated alone with GSH and CDNB. The same blank was used for every inhibitor concentration in each experiment.
Curcumin’s inhibitory potency on GSTs
Curcumin’s inhibitory potency was predicted using an in silico docking simulation with software (e.g., AutoDock Vina version 1.1.2).29 The free binding energy between different GST isoforms (namely GSTA1, GSTM1 and GSTP1) and curcumin was predicted (data not shown). Then, the constant of inhibition Ki was calculated using Equation 3.
Equation 3:
where ∆G is the free binding energy found using the in silico analysis, R is the gas constant of 1.987 cal*K-1*mol-1, and T is the temperature during the experiment, in this case in Kelvin (298 K).
Based on the free binding energy results returned by the AutoDock Vina, curcumin is a potent GST inhibitor of human GSTA1, GSTM1 and GSTP1, with Ki values of 78.1 µM, 78.1 µM and 27.4 µM respectively. The first inhibitory assays were conducted using these computational Ki estimations, and the concentrations were adjusted according to the results, if necessary.
First, the IC50 was estimated. This characteristic is the concentration of inhibitor that reduces an enzyme’s reaction rate by 50%. It is therefore dependent on the enzyme concentration30. Nine final concentrations of the inhibitor were prepared, ranging from 0.39 µM to 100 µM with each concentration double that of the previous one. The assay solution was composed of 20 µL of GSH 2.5 mM, 20 µL of GST from equine liver at 0.01 U/mL final, 2 µL of the curcumin solution, and 148 µL of PBS. The control was composed of the same solution with the diluent used for curcumin. This mixture was incubated for 15 minutes to begin the enzyme assay and to avoid degradation of the curcumin in the assay solution, as this compound is unstable in buffer solutions31. Next, 10 µL of CDNB solution at 4 mM was added to each well, for a final concentration of 0.2 mM CDNB. Absorbance at 340 nm was recorded every minute for 10 minutes, to calculate changes in absorbance per minute. To calculate the IC50, each sample incubated with curcumin was normalized to the control, to give the percentage of activity. These results were analyzed using the plotting software by calculating the non-linear regression of the logarithmic concentration of the inhibitor versus the inhibition. The IC50 found for curcumin on GST from equine liver was 31.6 ± 3.6 µM (Figure 3A).
The same experiment was conducted in parallel using ethacrynic acid as a positive control, with concentrations ranging from 0.39 to 100 µM, as with curcumin. The IC50 was found to be 6.6 ± 1.1 µM (Figure 3B).
The next step was to assess curcumin’s mode of inhibition on these transferases. Four different concentrations of curcumin were tested: 0, 7.5, 15, and 30 µM, along with four different concentrations of CDNB: 0.2, 0.4, 1, and 1.5 mM. The protocol was the same as for the previous experiment. Each condition’s velocity was calculated using Equation 2. A curve was plotted for each inhibitor concentration, with the velocity (in µM/min) against the substrate concentration (mM) (Figure 3C). The Vmax and Km were determined based on each plot with GraphPad Prism. The potential inhibitor’s mode of inhibition was assessed according to the changes in these two parameters and the assays’ different condition.24 As Vmax did not significantly change between conditions but Km increased with the different inhibitor concentrations, curcumin’s inhibition of GSTs is competitive. This pattern of inhibition occurs when the inhibitor competes with the substrate for the active site of the enzyme. To measure the Ki of a competitive inhibitor, GraphPad uses Equation 4 and Equation 5 as described by Copeland et al.32.
Equation 4:
Equation 5:
where Km obs is the observed Michaelis–Menten constant, Km is the Michaelis–Menten constant of the control, [I] is the inhibitor concentration, Ki is the constant of inhibition, Y is the velocity, and X is the substrate concentration.
The calculations are based on the same experiments as the assessment of the mode of inhibition. The Ki estimated for curcumin’s inhibition of GST from equine liver was 23.2 ± 3.2 µM.
Figure 1: Flowchart describing the steps of the GST enzymatic and inhibition assays. Details about the procedure are presented in the protocol section. Please click here to view a larger version of this figure.
Figure 2: Michaelis-Menten plot of GST enzyme activity. Increased concentration of the substrate CDNB were used with a fixed concentration of GSH (2.5 mM) to determine the GST activity from a pool of GST from equine liver. Km value for CDNB was determined as 0.26 ± 0.08 mM according to the curve of the graph. Each data point on the plot represents the mean of four different experimental runs with SD. Please click here to view a larger version of this figure.
Figure 3: Effect of curcumin and ethacrynic acid on GST enzyme activity. (A-B) Addition of increasing concentrations of either curcumin (A) or the positive control ethacrynic acid (B), whilst keeping the same GST (0.01 U/mL), GSH (2.5 mM) and CDNB (0.2 mM) final concentrations were used to calculate the IC50 of both compounds for GST from equine liver with a non-linear regression. IC50 was determined as 31.6 ± 3.6 µM for curcumin and 6.6 ± 1.1 µM for ethacrynic acid. (C) Michaelis–Menten plot of different concentrations of curcumin, tested against varying concentrations of CDNB substrate indicating a competitive mode of inhibition. With no inhibitor, Vmax and Km were measured as 132.7 ± 4.6 µM and 0.5 ± 0.08 µM, respectively. With 30 µM of curcumin, Vmax and Km were 130.4 ± 13.1 µM and 1.08 ± 0.39 µM. Data points in all graphs (A-C) represent the means of three different experimental runs with SD. Please click here to view a larger version of this figure.
We provide a protocol describing each step of a spectrophotometric GST enzyme assay, to screen putative inhibitors (Figure 1, Table 1) and quantify their inhibitory potency. We also emphasized the most crucial criteria to consider for accurate enzyme assays providing reproducible results. Major advantages of the described protocol over other colorimetric methods or mass spectrometry is that this protocol is quick and easy to perform and provide quantitative measurements of the GST activity as well as the inhibition potency of screened molecules.
We present the way of calculating the two most important Michaelis–Menten parameters of an enzyme inhibitor: the IC50 and the Ki. A potent inhibitor will exert the lowest possible Ki and IC50, indicating that the affinity between the inhibitor and the enzyme is high30. As IC50 is dependent upon the enzyme concentration and assay conditions33, it may be difficult to use this value to compare inhibitors from different experiments or obtained using other assay conditions34. Using the constant of inhibition Ki is a better indicator of the inhibitory potency of potential compounds. Ki can be used to compare two inhibitors with different modes of inhibition, as it relies solely on the affinity between the inhibitor and the enzyme. However, to have a clear idea of the nature of the inhibition one must determine both of the inhibitor’s parameters30. We measured curcumins’ IC50 and Ki as 31.6 ± 3.6 µM and 23.2 ± 3.2 µM, respectively, indicating that this compound is a potent GST inhibitor. These results confirmed the in silico predictions, that estimated the Ki values between 27.4 to 78.1 µM for different human GST isoforms and curcumin.
Enzymatic activity or rate of reaction and amount of enzyme
As stated above, IC50 is dependent on the enzyme concentration, and conducting an experiment with an unknown level of enzymatic activity might lead to false conclusions33. To control for other factors, which might reduce inhibitory activity, one should consider and measure enzymatic activity for each new batch of GSTs. For instance, degradation of an enzymatic batch, caused by too many freeze/thaw cycles, might decrease the activity and thus lead to a lower IC50 even if the experiment was run under the same conditions. In other words, using 0.01 units of enzyme will not give the same results as using 1 unit. Using too many enzymes might lead to a rapid depletion of the substrate and the reaction will not have a linear shape. This parameter could thus lead to inexact result, as no change in absorbance would be seen after a long incubation time.
Km Value
To ensure the best conditions for assessing the type of inhibition exhibited by an inhibitor, the substrate concentration must be equal to or below the Michaelis–Menten constant (Km). Km is represented by the concentration of substrate required to occupy half of the active sites on the enzyme28. For instance, higher substrate concentration can counteract a competitive inhibitor and assessing this type of inhibition will be difficult in such setting. Thus, one of the crucial steps in this methodology is the determination of the enzyme’s Km for the selected substrate (here CDNB). In some studies, this value was not determined and this could lead to false conclusions about the pattern of inhibition caused by the inhibitor, and, if the mode of inhibition is incorrect, the Ki will be calculated incorrectly as the equation relies on the pattern of inhibition26,28. If a different GST isoform is tested, a new assessment of the Km value is mandatory, as this value is unique for a pair of enzyme and ligand. As we used a concentration of CDNB slightly lower than the Km (0.2 mM), that we defined as 0.26 ± 0.08 mM, the predicted competitive mode of inhibition of curcumin on GST was accurately determined.
IC50
Obtaining a good sigmoidal curve with which to estimate the IC50 requires finding both the bottom and top plateaus. The bottom plateau represents the concentrations of an inhibitor that provide the maximum inhibitory activity. In some cases, a compound might not inhibit the enzyme fully, even at high concentrations, because of such technical issues as solubility. However, tools like GraphPad Prism can fit the bottom plateau quite accurately. The top plateau is made up of concentrations of the inhibitor, which are insufficient to inhibit the enzyme, and therefore the activity is maximal. Both these plateaus are crucial to determining the IC50 as well as concentrations in between, in order to find the slope of the curve—then the IC50 can be derived from the shape of the sigmoid curve35. Curcumin is poorly soluble in water, thus the maximum concentration used in this assay is limited, to avoid precipitation in the assay solution. Thus, less concentrated solutions, which does not inhibit fully the GST activity, were used. This raised issues for the determination of the bottom plateau. To counter this problem, we predicted bottom values based on the non-linear regression graph, which provided an IC50 of 31.6 ± 3.6 µM for curcumin (Figure 3A). For ethacrynic acid, there was no need to predict the values for the bottom plateau as this compound is soluble in the assay solution and the IC50 was measured at 6.6 ± 1.1 µM.
This method can be applied to the most expressed GST isoforms in human, namely GSTA1, GSTM1 or GSTP1. However, this protocol is not suited to quantify the activity of the GSTT1 isoform, as CDNB is not a substrate for this subtype.36, 37 Meanwhile, the protocol can be slightly modified to counter this issue. For instance , using 1,2-epoxy-3-(4’-nitrophenoxy)propane (ENPP) as a substrate for GSTT1 and measure the quantity of conjugate at 360nm instead of 340nm.37
Steps of protocol can be the adapted and applied to test GST activity and inhibitor testing in cell culture experiments. Measurement of the GST activity on cells treated with and without a GST inhibitor will indicate if this compound can be used in such experimental setting. It is especially interesting when the inhibitor is lipophilic. For instance, we presented that curcumin is a potent GST inhibitor using this protocol. Nevertheless, its application to cellular studies may be limited, as the molecule is poorly soluble in water and degrades quickly in medium.31 Another amelioration of this protocol is possible regarding the non-isoform specificity of the substrate CDNB. Using this protocol in cell studies will only give information about the overall GST activity, but not on the activity of precise GST subtype. Adding isoform-specific substrate and/or using specific recombinant GST isoform will permit to test isoform specific GST inhibitors.
To conclude, we describe a complete procedure to test GST inhibitors that have potential to be used in combination with electrophilic chemotherapy. We emphasized the crucial steps of a GST enzyme and inhibition assay, to test potential interesting molecule and determine their efficiency as inhibitor with quantitative values, the IC50 and the Ki. This method can be applied to any putative compound and performed on the most expressed human GST isoforms (GSTA1, GSTM1 and GSTP1), or slightly adapted to perform cell culture studies with GST inhibitors or measure the activity of other interesting GST isoform of choice.
Reagents | Name | Concentration in the assay solution | 其他 |
Substrate | CDNB | Measured Km (mM) | Diluted in 95% ethanol. The final ethanol concentration should be ≤ 5% (v/v) |
Conjugating substrate | GSH | 2.5 mM | Diluted in water. |
The concentration must saturate the solution. | |||
Buffer | PBS | – | pH = 7.1 |
Enzyme | Pool of GST isoforms or pure isoform | 0.01 unit/mL, determined experimentally. | Diluted in water. |
GST inhibitor | Potential compound of choice | IC50: 3 concentrations that maximally inhibit, 3 that minimally inhibit, and 3 in between. | Diluted in DMSO and then in water, to have a final concentration of DMSO ≤ 1% (v/v) |
Ki: 3 concentrations around the IC50. | |||
Parameters | |||
Room temperature (25°C) | |||
pH = 7.1 | |||
DMSO ≤ 1% (v/v) | |||
Ethanol ≤ 5% (v/v) |
Table 1: Summary of the reagents and parameters to consider during a GST inhibition assay.
The authors have nothing to disclose.
This research work was supported by the CANSEARCH Foundation. The authors would like to acknowledge Ms. Laurence Lesne and Mr. Yoann Sarmiento for technical assistance, especially in conducting the replicating experiments while standardizing the assays with inhibitors. Fruitful discussions and inputs by Mr. Denis Marino, Dr. Simona Mlakar and Dr. Vid Mlakar are greatly acknowledged. We thank Dr.Patricia Huezo-Diaz Curtis for her help with the narration of the video. We thank Dr. Muthukumar for his inputs on in silico predictions. We also thank Mr. Darren Hart for his help in English language proof reading of this manuscript.
1-Chloro-2,4-dinitrobenzene (CDNB) | Sigma-Aldrich | 237329 | Substrate used for the GST enzymatic assay |
Corning UV-Transparent Microplates | Sigma-Aldrich | CLS3635 | Transparent plate to perform the enzymatic assay. When using 200 ul, the pathlength is 0.552 cm for this plate. |
Curcumin | Sigma-Aldrich | 8511 | Used for the results section, to test the inhibition potency of curcumin |
Dimethyl sulfoxide (DMSO) | Sigma-Aldrich | D2650 | To prepare the stock of the putative inhibitor |
DPBS | Sigma-Aldrich | D8537 | Buffer for the enzymatic reaction |
Ethanol 95% | Fisher scientific | 10542382 | To dilute the CDNB |
Glutathione S-Transferase from equine liver | Sigma-Aldrich | G6511 | Used for the results section, to test the inhibition potency of curcumin |
L-glutathione reduced (GSH) | Sigma-Aldrich | G4251 | Co-substrate for the GST enzymatic assay |
Pierce BCA Protein Assay Kit | ThermoFisher | 23225 | To quantify the amount of protein present in the enzymatic solution |
Spectramax iD3 | Molecular devices | To do spectrophotometric measurments |