Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
Published: October 31, 2023
Abstract
Source: Trevaskis, N. L., et al. The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport. J. Vis. Exp. (2015).
This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Cannulation of the Mesenteric Lymph Duct Place the animal with its right side facing toward the operator. Perform the surgery with or without the assistance of a surgical microscope as required. Open the top layer of the abdominal muscle wall with a straight 4 cm incision extending from the midline (xiphoid pr…
Representative Results
Figure 1. Photographs of (A) the abdomen shaved in preparation to cannulate the lymph duct, (B) the abdominal muscle wall opened with a straight 4 cm incision extending from the midline to the right flank to access the lymph duct, and (C) the superior mesenteric lymph duct (in yellow circle) perpendicular to the right kidney.
Disclosures
The authors have nothing to disclose.
Materials
| Sterile saline | Baxter healthcare | AHB 1307 | Any brand can be used. Example here is Baxter 100 ml saline bags, box of 50 |
| 70 % ethanol in water | Any | Any brand can be used | |
| Chlorhexidine gluconate solution (Microshield 4) | Livingstone International | JJ60243L | Any brand can be used. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =JJ60243L |
| Betadine solution | Livingstone International | BU0510 | Any brand can be used. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =BU0510 |
| Ilium Ketamil (Ketamine 100 mg/ ml) | PROVET VICTORIA | KETA I 1 | http://www.provet.vom.au |
| Heparin (35000I.U. in 35 mL | SigmaPharmaceuticals | 337220 | http://www.sigmaco.com.au |
| Polyethylene (PE) cannula o.d. 0.96 mm x i.d. 0.58 mm | Microtube extensions | PE8050 | Any brand can be used. Example here is PE tubing 0.96 x0.58 mm, 30 m |
| Polyethylene (PE) cannula o.d. 0.8 mm x i.d. 0.5 mm |
Microtube extensions | PE9658 | Any brand can be used. Example here is PE tubing 0.8 x0.5 mm, 30 m |
| Markers | Any | Any brand can be used | |
| Veterinary adhesive | Any | Any brand can be used | |
| 23 gauge needles | Livingstone International | DN23GX0.75LV | Any brand can be used. Example here is Livingstone Disposable Needle, Sterile, 23GX0.75inch, 100/BOX. http:// www.livingstone.com.au/? PG=search_result&CAT= 6&search=DN23GX0.75LV |
| 25 gauge needles | Livingstone International | DN25GX1.0LV | Any brand can be used. Example here is Livingstone Disposable Needle, Sterile, 25GX1.0inch, 100/BOX. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search= DN25GX1.0LV |
| 1 ml syringe | Livingstone International | T3SS01TA | Any brand can be used. Example here is Terumo syringe 1 ml Slip Tuberculin 100/Box. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =T3SS01TA |
| 10 ml syringe | Livingstone International | T3SS10SA | Any brand can be used. Example here is Terumo syringe 10 ml Slip 100/Box. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =T3SS10SA |
| Gauze swabs | Livingstone International | GSC075 | Any brand can be used and cut to required size. Example here is gauze swabs cotton filled 7.5×7.5 cm, 8 ply. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =GSC075 |
| Heating pad | Ratek | WT1 | Any brand that keeps temperature at 37C can be used. Example here is Ratek warming tray. |
| Surgical microscope | Zeiss | 495005-0014-000 | Any brand can be used. Example here is Zeiss Stereomicroscope Stemi 2000-C with Stand S Double Spot and KL 300 LED. https:// www.micro-shop.zeiss.com/? l=en&p=us&f=e&i=10143 |
| 1 x Small surgical scissors | Fine Science Tools (FST) | 14060-09 | Any brand can be used. Example here is FST Fine Scissors, 9 cm with 21 mm cutting edge, sharp, straight. http://www.finescience.ca/ Special-Pages/Products.aspx? ProductId=40&CategoryId=17 |
| 2 x Forceps with serrated curved tip | Fine Science Tools (FST) | 11001-13 | Any brand can be used. Example here is FST 13 cm standard pattern forceps with curved 2.8×1.4 mm tip. http://www.finescience.ca/ Special-Pages/Products.aspx? ProductId=405&CategoryId=32 |
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Cite This Article
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid. J. Vis. Exp. (Pending Publication), e21759, doi: (2023).