Locating the Cisterna Magna in a Pig Model: A Surgical Method to Access the Cisterna Magna in a Pig Model for Direct Cannulation

Published: April 30, 2023

Abstract

Source: Bèchet, N. B., et al. Direct Cannula Implantation in the Cisterna Magna of Pigs. J. Vis. Exp. (2021).

In this video, we describe a method to access the cisterna magna in a pig model. The cisterna magna is a preferred site for studies concerning cerebrospinal fluid.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animal Preparation Sedate a pig by intramuscular (i.m.) injection of tiletamine (3.75 mg/kg) and zolazepam (3.75 mg/kg) and dexmedetomidine (37.5 μg/kg). Wait for it to become unconscious. Prepare an intravenous line by inserting a 20 G cannula into the ear vein. NOTE: Make sure the cannula is in t…

Representative Results

Figure 1. Cisterna magna cannulation in pigs. (A) Pig prepped prior to the start of the surgery and marked where dermal incisions will be performed starting from the occipital crest (OC) then posterior to thoracic vertebrae (TV) and lateral to each ear base (EB). (B) Head in the relaxed position with the trapezius, semispinalis capitus biventer and semi…

Disclosures

The authors have nothing to disclose.

Materials

Anatomical forceps NA NA
Marker pen NA NA
No. 21 Scalpel blade Agnthos BB521
No. 4 Scalpel handle Agnthos 10004-13
Self-retaining retractors NA NA
Surgical forceps NA NA

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Cite This Article
Locating the Cisterna Magna in a Pig Model: A Surgical Method to Access the Cisterna Magna in a Pig Model for Direct Cannulation. J. Vis. Exp. (Pending Publication), e20821, doi: (2023).

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