Zebrafish Rheotaxis Assay: A Method for Measuring Orientation Behavior in Response to Different Water Flow Rates
Published: April 30, 2023
Abstract
Source: Cresci, A., et al. Assessing the Influence of Personality on Sensitivity to Magnetic Fields in Zebrafish. J. Vis. Exp. (2019)
This video describes the rheotaxis assay in zebrafish. The method involves the measurement of orientation behavior of zebrafish in response to different water flow rates under the influence of different magnetic fields.
Protocol
1.Set up of the Magnetic Field with the One-dimensional Magnetic Field Manipulation Switch on the Power unit (Figure 1A). Place the coiled tunnel in the location where the rheotactic protocol will be performed (section 3) but keep it disconnected from the swimming apparatus (Figure 1A). Place a magnetic probe connected with a Gauss/Teslameter inside the tunnel and verify which voltage is necessary to obtain the chosen magnetic field value along the major axis of the…
Representative Results
Figure 1: Setup for magnetic field control. (A) Rendering of the swimming tunnel with a solenoid for the induction of a static, horizontal magnetic field within the tunnel. The solenoid (0.83 turns/cm) is connected to a power unit and it generates fields in the range of ±250 µT (intensity range that includes the earth’s magnetic field range). On the right-ha…
Materials
| 9500 G meter | FWBell | N/A | Gaussmeter, DC-10 kHz; probe resolution: 0.01 μT |
| AD5755-1 | Analog Devices | EVAL-AD5755SDZ | Quad Channel, 16-bit, Digital to Analog Converter |
| ALR3003D | ELC | 3.76024E+12 | DC Double Regulated power supply |
| BeagleBone Black | Beagleboard.org | N/A | Single Board Computer |
| Coil driver | Home made | N/A | Amplifier based on commercial OP (OPA544 by TI) |
| Helmholtz pairs | Home made | N/A | Coils made with standard AWG-14 wire |
| HMC588L | Honeywell | 900405 | Rev E Digital three-axis magnetometer |
| MO99-2506 | FWBell | 129966 | Single axis magnetic probe |
| Swimming apparatus | M2M Engineering Custom Scientific Equipment | N/A | Swimming apparatus composed by peristaltic pump and SMC Flow switch flowmeter with digital feedback |
| TECO 278 | TECO | N/A | Thermo-cryostat |
Cite This Article
Zebrafish Rheotaxis Assay: A Method for Measuring Orientation Behavior in Response to Different Water Flow Rates. J. Vis. Exp. (Pending Publication), e20170, doi: (2023).