Subcutaneous Dihydrotestosterone (DHT) Treatment: Mouse Model of Polycystic Ovary Syndrome (PCOS)

Published: April 30, 2023

Abstract

Source: Xue, P., et al. A Hyperandrogenic Mouse Model to Study Polycystic Ovary SyndromeJ. Vis. Exp. (2018).

This article describes a way to prepare and insert dihydrotestosterone (DHT) pellets to model Polycystic Ovary Syndrome (PCOS) in mice. The example protocol demonstrates the procedure in mice used for reproductive and metabolic studies.   

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Create PCOS-like Mouse Model NOTE: The mice used in this study were a mixed background (C57/B6, CD1, 129Sv) and were maintained with food and water ad libitum in a 14/10 h light/dark cycle at 24 °C. DHT Pellet Preparation A…

Disclosures

The authors have nothing to disclose.

Materials

Crystalline 5α-DHT powder  Sigma-Aldrich A8380-1G
Dow Corning Silastic tubing Fisher Scientific 11-189-15D 0.04in/1mm inner diameter x 0.085in/2.15mm outer diameter
Medical adhesive silicone Factor II, InC.  A-100
Goggles, lab coats, gloves and masks
3 mL Syring  Becton, Dickinson and Company (BD) 30985
Attached needle: 20G BD 305176
Ruler: any length than 10 cm with milimeter scale. 
Xylazine  Vet one AnnSeA LA, MWI, Boise NDC13985-704-10 100 mg/mL
Ketamine Hydrochloride Hospira, Inc NDC 0409-2051-05 100 mg/mL
Surgical staple AutoClip® System, Fine Science Tool 12020-00
Insulin syringe BD 329461 1/2 CC, low dose U-100 insulin syringe
Trocar  Innovative Research of America MP-182
Razor blade Fisher Scientific 12-640
Clidox Fisher Scientific NC0089321
Surgical underpad Fisher Scientific 50587953 Andwin Scientific 56616018
Betadine Antiseptic Solution Walgreens
3M Vetbond (n-butyl cyanoacrylate) 3M Science. Applied to Life

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Cite This Article
Subcutaneous Dihydrotestosterone (DHT) Treatment: Mouse Model of Polycystic Ovary Syndrome (PCOS). J. Vis. Exp. (Pending Publication), e20014, doi: (2023).

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