JOVE JOURNAL

遗传学

Showing 337 - 348 in 413 videos.

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

Rizwan Rehimi¹, Michaela Bartusel¹, Francesca Solinas², Janine Altmüller², Alvaro Rada-Iglesias^1,3

¹Center for Molecular Medicine Cologne (CMMC),University of Cologne, ²Cologne Center for Genomics (CCG),University of Cologne, ³Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Research Center and Systems Biology of Ageing Cologne,University of Cologne

Journal (Genetics)

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Natalia Rivera-Torres^1,2, Eric B. Kmiec^1,2

¹Gene Editing Institute, Helen F. Graham Cancer Center and Research Institute,Christiana Care Health Services, ²Department of Medical Sciences,University of Delaware

Journal (Genetics)

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Dhaval S Patel*¹, Giovanni Diana*¹, Eugeni V. Entchev¹, Mei Zhan^2,3,4, Hang Lu^2,3,4, QueeLim Ch'ng¹

¹Centre for Developmental Neurobiology,King's College London, ²Interdisciplinary Bioengineering Graduate Program,Georgia Institute of Technology, ³Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology, ⁴School of Chemical & Biomolecular Engineering,Georgia Institute of Technology

Journal (Genetics)

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Stephen D. Willis¹, A. K. M. Nawshad Hossian², Nathan Evans², Mark J. Hickman²

¹Department of Molecular Biology,Rowan School of Osteopathic Medicine, ²Department of Biological Sciences,Rowan University

Journal (Genetics)

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Marisa E. Miller*^1,2, Katie L. Liberatore*^1,3, Shahryar F. Kianian^1,3

¹Cereal Disease Laboratory,United States Department of Agriculture-Agricultural Research Service, ²Department of Horticultural Science,University of Minnesota, ³Department of Plant Pathology,University of Minnesota

Journal (Genetics)

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer’s Disease Gene Susceptibility Assessment

Aymric Kisserli^1,2, Thierry Tabary^1,2, Jacques Henri Max Cohen^1,2, Valérie Duret^1,2, Rachid Mahmoudi^3,4

¹Department of Immunology,Reims University Hospitals, Robert Debré Hospital, ²Faculty of Medicine, LRN EA 4682,University of Reims Champagne-Ardenne, ³Department of Internal Medicine and Geriatrics,Reims University Hospitals, Maison Blanche Hospital, ⁴Faculty of Medicine, EA 3797,University of Reims Champagne-Ardenne

Journal (Genetics)

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Pingyu Zhang*¹, Andres Rojas*¹, Boris Blechacz¹

¹Department of Gastroenterology, Hepatology and Nutrition,The University of Texas MD Anderson Cancer Center

Journal (Genetics)

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Keiji Hirai¹, Hiromichi Yoshizawa², Toshimi Imai², Yusuke Igarashi², Ichiro Hirahara², Susumu Ookawara², Kenichi Ishibashi³, Yoshiyuki Morishita¹

¹Division of Nephrology, First Department of Integrated Medicine,Saitama Medical Center, Jichi Medical University, ²Division of Nephrology, Department of Internal Medicine,Jichi Medical University, ³Department of Medical Physiology,Meiji Pharmaceutical University

Journal (Genetics)

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Mohamed I. Hassan*¹, Fern R. McSorley*¹, Kinya Hotta², Christopher N. Boddy¹

¹Department of Chemistry and Biomolecular Sciences,University of Ottawa, ²School of Biosciences,The University of Nottingham Malaysia Campus

Journal (Genetics)

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

Bang Shen¹, Robin H. Powell², Michael S. Behnke²

¹State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine,Huazhong Agricultural University, ²Pathobiological Sciences, School of Veterinary Medicine,Louisiana State University

Journal (Genetics)

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

Benedetto Daniele Giaimo¹, Francesca Ferrante¹, Tilman Borggrefe¹

¹Institute of Biochemistry,University of Giessen

Journal (Genetics)

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Erin L. Sternburg¹, Kristen C. Dias¹, Fedor V. Karginov¹

¹Cell Biology and Neuroscience,University of California, Riverside

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