JOVE JOURNAL

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Journal (Genetics)

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Joonsun Lee*¹, Min-hee Jung*¹, Euihwan Jeong*¹, Jungjoon K. Lee¹

¹ToolGen

Journal (Genetics)

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Ranjan K. Behera¹, Kevin D. Mlynek¹, Matthew S. Linz¹, Shaun R. Brinsmade¹

¹Department of Biology,Georgetown University

Journal (Genetics)

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Stefan Radtke¹, Anai M. Perez¹, Rasika Venkataraman¹, Sowmya Reddy¹, Kevin G. Haworth¹, Olivier Humbert¹, Hans-Peter Kiem^1,2,3, Christopher W. Peterson^1,2

¹Stem Cell and Gene Therapy Program,Fred Hutchinson Cancer Research Center, ²Department of Medicine,University of Washington, ³Department of Pathology,University of Washington

Journal (Genetics)

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Shaleen Shrestha*¹, Xing Liu*¹, Clarissa Stephanie Santoso*¹, Juan Ignacio Fuxman Bass^1,2

¹Department of Biology,Boston University, ²Bioinformatics Program,Boston University

Journal (Genetics)

Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins

Isabell-Christin Fiedler¹, Arne Weiberg², Karina van der Linde¹

¹Department of Biology,Regensburg University, ²Department of Biology,Ludwig-Maximilians University of Munich

Journal (Genetics)

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Hazuki Takahashi*¹, Harshita Sharma*¹, Piero Carninci^1,2

¹Laboratory for Transcriptome Technology,RIKEN Center for Integrative Medical Sciences, ²TransSINE Technologies Co. Ltd.

Journal (Genetics)

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Jingxin Wang¹, John Hammond², Kristen A. Johnson¹

¹Calibr,The Scripps Research Institute, ²Department of Integrative Structural and Computational Biology,The Scripps Research Institute

Journal (Genetics)

Determining 3′-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

Darja Pollpeter¹, Andrew Sobala¹, Michael H. Malim¹

¹Department of Infectious Diseases, School of Immunology & Microbial Sciences,King's College London

Journal (Genetics)

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Peggy Paschke¹, David A. Knecht², Thomas D. Williams¹, Peter A. Thomason³, Robert H. Insall³, Jonathan R. Chubb^4,5, Robert R. Kay¹, Douwe M. Veltman¹

¹MRC Laboratory of Molecular Biology, ²Department of Molecular and Cell Biology,University of Connecticut, ³Cancer Research UK Beatson Institute Glasgow, ⁴MRC Laboratory for Molecular Cell Biology,University College London, ⁵Department of Cell and Developmental Biology,University College London

Journal (Genetics)

Assessing Stem Cell DNA Integrity for Cardiac Cell Therapy

Jessica M. Miller*¹, Nikhil Maneesh Mardhekar*¹, Vasanthi Rajasekaran¹, Jianyi Zhang¹, Ram Kannappan¹

¹Department of Biomedical Engineering, School of Engineering, School of Medicine,University of Alabama at Birmingham

Journal (Genetics)

Novel Sequence Discovery by Subtractive Genomics

Kathryn C. Asalone¹, Megan M. Nelson¹, John R. Bracht¹

¹Biology Department,American University

Journal (Genetics)

Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells

Laura Avogaro¹, Claudio Oss Pegorar¹, Nicole Bettin^1,2, Emilio Cusanelli¹

¹Laboratory of Cell Biology and Molecular Genetics, Department of Cellular, Computational and Integrative Biology - CIBIO,University of Trento, ²Department of Life Sciences,University of Trieste

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