JOVE JOURNAL

生物学

Showing 145 - 156 in 3,291 videos.

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Journal (Biology)

Tissue Clearing of Mouse Kidneys for Confocal Microscopy

Lin Bai^1,2, Yaping Wu², Wenshu Dai¹, Qiuxiao Shi², Lei Wu², Jie Zhang^1,2, Lily Zheng³

¹Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital,Sichuan University, ²Core Facility of West China Hospital,Sichuan University, ³Regenerative Medicine Research Center, West China Hospital,Sichuan University

Journal (Biology)

Confocal and Light Sheet Fluorescence Microscopy (LSFM) Imaging of Cleared Mouse Kidneys

Lin Bai^1,2, Yaping Wu², Wenshu Dai¹, Qiuxiao Shi², Lei Wu², Jie Zhang^1,2, Lily Zheng³

¹Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital,Sichuan University, ²Core Facility of West China Hospital,Sichuan University, ³Regenerative Medicine Research Center, West China Hospital,Sichuan University

Journal (Biology)

Analysis of the GFP-Positive and mCherry-Positive Cells by Flow Cytometry

Lu-Ping Zhang¹, Yong-Hong Nie¹, Tuo Tang¹, Ai-Xue Zheng¹, Xian Hong¹, Tao Wang¹

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Journal (Biology)

Data Analysis of Flow Cytometry Results from Transfected HEK-293T Cells

Lu-Ping Zhang¹, Yong-Hong Nie¹, Tuo Tang¹, Ai-Xue Zheng¹, Xian Hong¹, Tao Wang¹

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Journal (Biology)

Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems

Lu-Ping Zhang¹, Yong-Hong Nie¹, Tuo Tang¹, Ai-Xue Zheng¹, Xian Hong¹, Tao Wang¹

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Journal (Biology)

Preparation and Transfection of HEK-293T Cells for FACS Sorting Analysis

Lu-Ping Zhang¹, Yong-Hong Nie¹, Tuo Tang¹, Ai-Xue Zheng¹, Xian Hong¹, Tao Wang¹

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Journal (Biology)

In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells

Ines Cherkaoui¹, Qian Du², Dieter Egli², Shivani Misra^1,3, Guy A. Rutter^1,4,5

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center,Columbia University, ³Department of Diabetes,Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine,Université de Montréal, ⁵Lee Kong Chian Imperial Medical School,Nanyang Technological University

Journal (Biology)

Optimized Protocol for Generating Functional Pancreatic Insulin-secreting Cells from Human Pluripotent Stem Cells

Ines Cherkaoui¹, Qian Du², Dieter Egli², Shivani Misra^1,3, Guy A. Rutter^1,4,5

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center,Columbia University, ³Department of Diabetes,Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine,Université de Montréal, ⁵Lee Kong Chian Imperial Medical School,Nanyang Technological University

Journal (Biology)

Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation

Kole H. Buckley¹, Keely A. Beyries¹, Sandra Ryeom², Sam S. Yoon², Bryson W. Katona¹

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

Journal (Biology)

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium

Kole H. Buckley¹, Keely A. Beyries¹, Sandra Ryeom², Sam S. Yoon², Bryson W. Katona¹

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

Journal (Biology)

Embedding Gastric Biopsy-Derived Single Cells in a Basement Membrane Matrix Dome for Organoid Generation

Kole H. Buckley¹, Keely A. Beyries¹, Sandra Ryeom², Sam S. Yoon², Bryson W. Katona¹

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

Journal (Biology)

Routine Passaging of Patient-Derived Gastric Organoids Through Fragmentation

Kole H. Buckley¹, Keely A. Beyries¹, Sandra Ryeom², Sam S. Yoon², Bryson W. Katona¹

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

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