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JOVE JOURNAL
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Showing 1,777 - 1,788 in 3,287 videos.
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Journal (Biology)
Subcloning Plus Insertion (SPI) – A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Thimma R. Reddy
1
,
Emma J. Kelsall
1
,
Léna M.S. Fevat
2
,
Sarah E. Munson*
3
,
Shaun M. Cowley*
1
1
Department of Biochemistry
,
University of Leicester
,
2
Center for Fisheries, Environment and Aquaculture Sciences
,
3
ES Cell Facility, Centre for Core Biotechnology Services
,
University of Leicester
Author
Thimma R. Reddy
1
,
Emma J. Kelsall
1
,
Léna M.S. Fevat
2
,
Sarah E. Munson*
3
,
Shaun M. Cowley*
1
机构
1
Department of Biochemistry
,
University of Leicester
,
2
Center for Fisheries, Environment and Aquaculture Sciences
,
3
ES Cell Facility, Centre for Core Biotechnology Services
,
University of Leicester
AUTHORS
Journal (Biology)
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in
Escherichia coli
Louise E. Bird
1
,
Heather Rada
1
,
Anil Verma
1
,
Raphael Gasper
2,3
,
James Birch
4
,
Matthew Jennions
4
,
Jan Lӧwe
3
,
Isabel Moraes
4
,
Raymond J. Owens
1
1
Oxford Protein Production Facility
,
Research Complex at Harwell
,
2
Protein Crystallography Group
,
Ruhr University
,
3
MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus
,
4
Membrane Protein Laboratory
,
Diamond Light Source
Author
Louise E. Bird
1
,
Heather Rada
1
,
Anil Verma
1
,
Raphael Gasper
2,3
,
James Birch
4
,
Matthew Jennions
4
,
Jan Lӧwe
3
,
Isabel Moraes
4
,
Raymond J. Owens
1
机构
1
Oxford Protein Production Facility
,
Research Complex at Harwell
,
2
Protein Crystallography Group
,
Ruhr University
,
3
MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus
,
4
Membrane Protein Laboratory
,
Diamond Light Source
AUTHORS
Journal (Biology)
Simple Method for Fluorescence DNA
In Situ
Hybridization to Squashed Chromosomes
Amanda M. Larracuente
1
,
Patrick M. Ferree
2
1
Department of Biology
,
University of Rochester
,
2
W. M. Keck Science Department
,
Claremont McKenna, Pitzer, and Scripps Colleges
Author
Amanda M. Larracuente
1
,
Patrick M. Ferree
2
机构
1
Department of Biology
,
University of Rochester
,
2
W. M. Keck Science Department
,
Claremont McKenna, Pitzer, and Scripps Colleges
AUTHORS
Journal (Biology)
Phenotyping Mouse Pulmonary Function
In Vivo
with the Lung Diffusing Capacity
Nathachit Limjunyawong
1
,
Jonathan Fallica
1
,
Amritha Ramakrishnan
2
,
Kausik Datta
3
,
Matthew Gabrielson
1
,
Maureen Horton
1
,
Wayne Mitzner
1
1
Department of Environmental Health Sciences
,
Johns Hopkins University Bloomberg School of Public Health
,
2
Department of International Health
,
Johns Hopkins University Bloomberg School of Public Health
,
3
Department of Medicine
,
Johns Hopkins University School of Medicine
Author
Nathachit Limjunyawong
1
,
Jonathan Fallica
1
,
Amritha Ramakrishnan
2
,
Kausik Datta
3
,
Matthew Gabrielson
1
,
Maureen Horton
1
,
Wayne Mitzner
1
机构
1
Department of Environmental Health Sciences
,
Johns Hopkins University Bloomberg School of Public Health
,
2
Department of International Health
,
Johns Hopkins University Bloomberg School of Public Health
,
3
Department of Medicine
,
Johns Hopkins University School of Medicine
AUTHORS
Journal (Biology)
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Daniel E. Bauer*
1,2,3
,
Matthew C. Canver*
1
,
Stuart H. Orkin
1,2,3,4
1
Harvard Medical School
,
2
Division of Hematology/Oncology
,
Boston Children's Hospital
,
3
Department of Pediatric Oncology
,
Dana-Farber Cancer Institute
,
4
Howard Hughes Medical Institute
Author
Daniel E. Bauer*
1,2,3
,
Matthew C. Canver*
1
,
Stuart H. Orkin
1,2,3,4
机构
1
Harvard Medical School
,
2
Division of Hematology/Oncology
,
Boston Children's Hospital
,
3
Department of Pediatric Oncology
,
Dana-Farber Cancer Institute
,
4
Howard Hughes Medical Institute
AUTHORS
Journal (Biology)
Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
Lin Zhang
1
,
Katleen Vranckx
2
,
Koen Janssens
2
,
Todd R. Sandrin
1
1
School of Mathematical and Natural Sciences
,
Arizona State University
,
2
Applied Maths NV
Author
Lin Zhang
1
,
Katleen Vranckx
2
,
Koen Janssens
2
,
Todd R. Sandrin
1
机构
1
School of Mathematical and Natural Sciences
,
Arizona State University
,
2
Applied Maths NV
AUTHORS
Journal (Biology)
In Vitro
Aggregation Assays Using Hyperphosphorylated Tau Protein
Dexin Sui*
1
,
Mengyu Liu*
1
,
Min-Hao Kuo
1
1
Department of Biochemistry and Molecular Biology
,
Michigan State University
Author
Dexin Sui*
1
,
Mengyu Liu*
1
,
Min-Hao Kuo
1
机构
1
Department of Biochemistry and Molecular Biology
,
Michigan State University
AUTHORS
Journal (Biology)
Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito
Aedes aegypti
Jackson T. Sparks
1
,
Joseph C. Dickens
1
1
Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Plant Sciences Institute, Invasive Insect Biocontrol and Behavior Laboratory
,
United States Department of Agriculture
Author
Jackson T. Sparks
1
,
Joseph C. Dickens
1
机构
1
Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Plant Sciences Institute, Invasive Insect Biocontrol and Behavior Laboratory
,
United States Department of Agriculture
AUTHORS
Journal (Biology)
Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Mark A. Baker
1
,
Louise Hetherington
1
,
Anita Weinberg
1
,
Tony Velkov
2
1
School of Environmental and Life Science
,
University of Newcastle
,
2
Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences
,
Monash University
Author
Mark A. Baker
1
,
Louise Hetherington
1
,
Anita Weinberg
1
,
Tony Velkov
2
机构
1
School of Environmental and Life Science
,
University of Newcastle
,
2
Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences
,
Monash University
AUTHORS
Journal (Biology)
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Yan Wei Lim
1
,
Matthew Haynes
2
,
Mike Furlan
1
,
Charles E. Robertson
3
,
J. Kirk Harris
4
,
Forest Rohwer
1
1
Department of Biology
,
San Diego State University
,
2
DOE Joint Genome Institute
,
3
Department of Molecular, Cellular and Developmental Biology
,
University of Colorado
,
4
Department of Pediatrics, School of Medicine
,
University of Colorado
Author
Yan Wei Lim
1
,
Matthew Haynes
2
,
Mike Furlan
1
,
Charles E. Robertson
3
,
J. Kirk Harris
4
,
Forest Rohwer
1
机构
1
Department of Biology
,
San Diego State University
,
2
DOE Joint Genome Institute
,
3
Department of Molecular, Cellular and Developmental Biology
,
University of Colorado
,
4
Department of Pediatrics, School of Medicine
,
University of Colorado
AUTHORS
Journal (Biology)
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Shubhra Majumder
1
,
Harold A. Fisk
1
1
Department of Molecular Genetics
,
The Ohio State University
Author
Shubhra Majumder
1
,
Harold A. Fisk
1
机构
1
Department of Molecular Genetics
,
The Ohio State University
AUTHORS
Journal (Biology)
Floral-Dip Transformation of Flax (
Linum usitatissimum
) to Generate Transgenic Progenies with a High Transformation Rate
Nasmah K. Bastaki
1
,
Christopher A. Cullis
1
1
Department of Biology
,
Case Western Reserve University
Author
Nasmah K. Bastaki
1
,
Christopher A. Cullis
1
机构
1
Department of Biology
,
Case Western Reserve University
AUTHORS
1
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