JOVE JOURNAL

生物学

Showing 97 - 108 in 3,237 videos.

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Journal (Biology)

Tissue Clearing of Mouse Kidneys for Confocal Microscopy

Lin Bai², Yaping Wu, Wenshu Dai, Qiuxiao Shi, Lei Wu, Jie Zhang², Lily Zheng

¹Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital,Sichuan University, ²Core Facility of West China Hospital,Sichuan University, ³Regenerative Medicine Research Center, West China Hospital,Sichuan University

Journal (Biology)

Confocal and Light Sheet Fluorescence Microscopy (LSFM) Imaging of Cleared Mouse Kidneys

Lin Bai², Yaping Wu, Wenshu Dai, Qiuxiao Shi, Lei Wu, Jie Zhang², Lily Zheng

¹Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital,Sichuan University, ²Core Facility of West China Hospital,Sichuan University, ³Regenerative Medicine Research Center, West China Hospital,Sichuan University

Journal (Biology)

Data Processing and Quantification of Glomeruli from Microscopy Images

Lin Bai², Yaping Wu, Wenshu Dai, Qiuxiao Shi, Lei Wu, Jie Zhang², Lily Zheng

¹Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital,Sichuan University, ²Core Facility of West China Hospital,Sichuan University, ³Regenerative Medicine Research Center, West China Hospital,Sichuan University

Journal (Biology)

Preparation and Transfection of HEK-293T Cells for FACS Sorting Analysis

Lu-Ping Zhang, Yong-Hong Nie, Tuo Tang, Ai-Xue Zheng, Xian Hong, Tao Wang

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Journal (Biology)

Analysis of the GFP-Positive and mCherry-Positive Cells by Flow Cytometry

Lu-Ping Zhang, Yong-Hong Nie, Tuo Tang, Ai-Xue Zheng, Xian Hong, Tao Wang

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Journal (Biology)

Analysis of Nonhomologous End Joining and Homologous Recombination Efficiency in HEK-293T Cells Using GFP-Based Reporter Systems

Lu-Ping Zhang, Yong-Hong Nie, Tuo Tang, Ai-Xue Zheng, Xian Hong, Tao Wang

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Journal (Biology)

Data Analysis of Flow Cytometry Results from Transfected HEK-293T Cells

Lu-Ping Zhang, Yong-Hong Nie, Tuo Tang, Ai-Xue Zheng, Xian Hong, Tao Wang

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Journal (Biology)

Optimized Protocol for Generating Functional Pancreatic Insulin-secreting Cells from Human Pluripotent Stem Cells

Ines Cherkaoui, Qian Du, Dieter Egli, Shivani Misra³, Guy A. Rutter^4,5

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center,Columbia University, ³Department of Diabetes,Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine,Université de Montréal, ⁵Lee Kong Chian Imperial Medical School,Nanyang Technological University

Journal (Biology)

In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells

Ines Cherkaoui, Qian Du, Dieter Egli, Shivani Misra³, Guy A. Rutter^4,5

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center,Columbia University, ³Department of Diabetes,Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine,Université de Montréal, ⁵Lee Kong Chian Imperial Medical School,Nanyang Technological University

Journal (Biology)

Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation

Kole H. Buckley, Keely A. Beyries, Sandra Ryeom, Sam S. Yoon, Bryson W. Katona

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

Journal (Biology)

Embedding Gastric Biopsy-Derived Single Cells in a Basement Membrane Matrix Dome for Organoid Generation

Kole H. Buckley, Keely A. Beyries, Sandra Ryeom, Sam S. Yoon, Bryson W. Katona

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

Journal (Biology)

Routine Passaging of Patient-Derived Gastric Organoids Through Fragmentation

Kole H. Buckley, Keely A. Beyries, Sandra Ryeom, Sam S. Yoon, Bryson W. Katona

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

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