JOVE JOURNAL

生物化学

Showing 649 - 660 in 778 videos.

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Myriam Jaraíz-Rodríguez¹, Ana González-Sánchez^1,2, Laura García-Vicente¹, Jose M. Medina¹, Arantxa Tabernero¹

¹Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular,Universidad de Salamanca, ²Centre for Cancer Research & Cell Biology (CCRCB), School of Medicine, Dentistry and Biomedical Sciences,Queen's University Belfast

Journal (Biochemistry)

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Genta Ito¹, Taisuke Tomita^1,2

¹Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, ²Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Journal (Biochemistry)

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Mayra A. Machuca^1,2, Anna Roujeinikova^1,2,3

¹Infection and Immunity Program,Monash Biomedicine Discovery Institute, ²Department of Microbiology,Monash University, ³Department of Biochemistry and Molecular Biology,Monash University

Journal (Biochemistry)

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Deborah A. Sarkes¹, Justin P. Jahnke¹, Dimitra N. Stratis-Cullum¹

¹Sensors and Electron Devices Directorate,US Army Research Laboratory

Journal (Biochemistry)

Protein Film Infrared Electrochemistry Demonstrated for Study of H₂ Oxidation by a [NiFe] Hydrogenase

Philip A. Ash*¹, Ricardo Hidalgo*¹, Kylie A. Vincent¹

¹Department of Chemistry,University of Oxford, Inorganic Chemistry Laboratory

Journal (Biochemistry)

Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics

Pavel Mokrejš¹, Matouš Huťťa¹, Jana Pavlačková², Pavlína Egner²

¹Department of Polymer Engineering, Faculty of Technology,Tomas Bata University in Zlín, ²Department of Fat, Tenside and Cosmetics Technology, Faculty of Technology,Tomas Bata University in Zlín

Journal (Biochemistry)

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Robert W. Harkness V¹, Philip E. Johnson², Anthony K. Mittermaier¹

¹Department of Chemistry,McGill University, ²Department of Chemistry,York University

Journal (Biochemistry)

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Anthony A. High¹, Haiyan Tan¹, Vishwajeeth R. Pagala¹, Mingming Niu^2,3, Ji-Hoon Cho¹, Xusheng Wang¹, Bing Bai², Junmin Peng^1,2

¹St. Jude Proteomics Facility,St. Jude Children's Research Hospital, ²Departments of Structural Biology and Developmental Neurobiology,St. Jude Children's Research Hospital, ³Heilongjiang University of Chinese Medicine

Journal (Biochemistry)

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

Thiago Cabral*^1,2,7,8, Marcus A. Toral*^3,4, Gabriel Velez^3,4, James E. DiCarlo^1,2, Anuradha M. Gore³, MaryAnn Mahajan³, Stephen H. Tsang^1,2, Alexander G. Bassuk^5,6, Vinit B. Mahajan^3,9

¹Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons,Columbia University, ²Edward S. Harkness Eye Institute,New York-Presbyterian Hospital, ³Omics Laboratory, Byers Eye Institute, Department of Ophthalmology,Stanford University, ⁴Medical Scientist Training Program,University of Iowa, ⁵Department of Pediatrics,University of Iowa, ⁶Department of Neurology,University of Iowa, ⁷Department of Ophthalmology,Federal University of Sao Paulo (UNIFESP), ⁸Department of Ophthalmology,Federal University of EspÍrito Santo (UFES), ⁹Palo Alto Veterans Administration, Palo Alto, CA

Journal (Biochemistry)

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Shilpy Joshi¹, Vanessa Ueberschlag-Pitiot¹, Daniel Metzger¹, Irwin Davidson¹

¹Department of Functional Genomics and Cancer,Institut de Génétique et de Biologie Moléculaire et Cellulaire

Journal (Biochemistry)

Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain

Ian Diner¹, Tram Nguyen¹, Nicholas T. Seyfried¹

¹Department of Biochemistry,Emory School of Medicine

Journal (Biochemistry)

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Sofie Nyström¹, Marcus Bäck¹, K. Peter R. Nilsson¹, Per Hammarström¹

¹IFM-Department of Chemistry,Linköping University

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