JOVE JOURNAL

生物化学

Showing 49 - 60 in 776 videos.

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Journal (Biochemistry)

Rhamnolipid Detection in Pseudomonas aeruginosa Supernatants Using TLC

Abigail González-Valdez¹, Jessica Hernández-Pineda², Gloria Soberón-Chávez¹

¹Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México, ²Departamento de Infectología e Inmunología,Instituto Nacional de Perinatología

Journal (Biochemistry)

Quantification of Rhamnolipids Present in the Biosurfactant of Pseudomonas aeruginosa

Abigail González-Valdez¹, Jessica Hernández-Pineda², Gloria Soberón-Chávez¹

¹Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México, ²Departamento de Infectología e Inmunología,Instituto Nacional de Perinatología

Journal (Biochemistry)

Detection and Quantification of Mono-Rhamnolipids and Di-Rhamnolipids Produced by Pseudomonas aeruginosa

Abigail González-Valdez¹, Jessica Hernández-Pineda², Gloria Soberón-Chávez¹

¹Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas,Universidad Nacional Autónoma de México, ²Departamento de Infectología e Inmunología,Instituto Nacional de Perinatología

Journal (Biochemistry)

Preparing HASM Cells for cAMP Detection Using Inverted Fluorescence Microscopy

Isabella Cattani-Cavalieri¹, Jordyn Margolis¹, Camelia Anicolaesei¹, Francisco J. Nuñez¹, Rennolds S. Ostrom¹

¹Department of Biomedical and Pharmaceutical Sciences,Chapman University School of Pharmacy

Journal (Biochemistry)

Inverted Fluorescence Microscopy and Data Analysis to Assess Drug Effects on cAMP in HASM Cells

Isabella Cattani-Cavalieri¹, Jordyn Margolis¹, Camelia Anicolaesei¹, Francisco J. Nuñez¹, Rennolds S. Ostrom¹

¹Department of Biomedical and Pharmaceutical Sciences,Chapman University School of Pharmacy

Journal (Biochemistry)

Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ

Isabella Cattani-Cavalieri¹, Jordyn Margolis¹, Camelia Anicolaesei¹, Francisco J. Nuñez¹, Rennolds S. Ostrom¹

¹Department of Biomedical and Pharmaceutical Sciences,Chapman University School of Pharmacy

Journal (Biochemistry)

Transformation of E. coli Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity

Hloniphani Rachel Ncube¹, Unarine Dali¹, Tendamudzimu Harmfree Dongola¹, Addmore Shonhai¹

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

Analysis of Hsp70 Chaperone Activity Using SDS PAGE and Western Blot Analyses of Transformed E. coli Protein

Hloniphani Rachel Ncube¹, Unarine Dali¹, Tendamudzimu Harmfree Dongola¹, Addmore Shonhai¹

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70

Hloniphani Rachel Ncube¹, Unarine Dali¹, Tendamudzimu Harmfree Dongola¹, Addmore Shonhai¹

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

James Sandy¹, Halina Mikolajek^1,2, Amy J. Thompson¹, Juan Sanchez-Weatherby¹, Michael A Hough^1,2

¹Diamond Light Source Ltd,Harwell Science and Innovation Campus, ²Research Complex at Harwell,Harwell Science and Innovation Campus

Journal (Biochemistry)

In Situ X-ray Crystallographic Data Collection Using the Beamline at Diamond Light Source

James Sandy¹, Halina Mikolajek^1,2, Amy J. Thompson¹, Juan Sanchez-Weatherby¹, Michael A Hough^1,2

¹Diamond Light Source Ltd,Harwell Science and Innovation Campus, ²Research Complex at Harwell,Harwell Science and Innovation Campus

Journal (Biochemistry)

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Russell Spencer-Smith^1,2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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