JOVE JOURNAL

生物化学

Showing 73 - 84 in 776 videos.

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Journal (Biochemistry)

Isothermal Titration Calorimetry for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Biolayer Interferometry (BLI) for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Surface Plasmon Resonance (SPR) for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

Trinity Cookis¹, Paul Sauer^2,3, Simon Poepsel^4,5, Bong-Gyoon Han⁶, Dominik A. Herbst^1,2,6, Robert Glaeser⁶, Eva Nogales^1,2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Designing Streptavidin Affinity Grids for Cryo-Electron Microscopy

Trinity Cookis¹, Paul Sauer^2,3, Simon Poepsel^4,5, Bong-Gyoon Han⁶, Dominik A. Herbst^1,2,6, Robert Glaeser⁶, Eva Nogales^1,2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Sample Preparation for In Situ Cryotomography of Mammalian Cells

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon

Anjelique Sawh-Gopal^1,2, Aygul Ishemgulova*¹, Eugene Y. D. Chua*¹, Mahira F. Aragon*¹, Joshua H. Mendez¹, Edward T. Eng¹, Alex J. Noble¹

¹Simons Electron Microscopy Center, New York Structural Biology Center, ²Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

Journal (Biochemistry)

Using Smart Leginon Autoscreen for CryoEM Grid Screening

Anjelique Sawh-Gopal^1,2, Aygul Ishemgulova*¹, Eugene Y. D. Chua*¹, Mahira F. Aragon*¹, Joshua H. Mendez¹, Edward T. Eng¹, Alex J. Noble¹

¹Simons Electron Microscopy Center, New York Structural Biology Center, ²Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

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