JOVE JOURNAL

生物化学

Showing 145 - 156 in 776 videos.

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Journal (Biochemistry)

Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts

Mingqing Tang^1,2, Hongjun Lin¹, Chengjia Hu¹, Hui Yan^1,2

¹Medicine School,Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

Journal (Biochemistry)

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Juliane Corrêa Glória^1,2,3, Yury Oliveira Chaves^2,3,4, Adélia Marques de Figueiredo¹, Caio Coutinho de Souza², Eliza Raquel Duarte da Silva², Jeniffer Clorives Lopes Batista^2,5, Paulo Afonso Nogueira^2,3,5, Luis André Morais Mariúba^1,2,3,5

¹Programa de Pós-Graduação em Biotecnologia da Universidade Federal do Amazonas - UFAM, ²Instituto Leônidas e Maria Deane (ILMD),Fundação Oswaldo Cruz (FIOCRUZ), ³Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro do Instituto Leônidas e Maria Deane (ILMD),Fundação Oswaldo Cruz (FIOCRUZ), ⁴Programa de Pós-graduação em Ciências Aplicadas à Hematologia – Universidade Estadual do Amazonas/Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas, ⁵Programa de Pós-Graduação em Imunologia Básica e Aplicada, Instituto de Ciências Biológicas,Universidade Federal do Amazonas (UFAM)

Journal (Biochemistry)

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Tenzin Tseyang*¹, Jonathan Valeros*¹, Paula Vo*¹, Jessica B. Spinelli^1,2

¹Program in Molecular Medicine,University of Massachusetts Chan Medical School, ²Cancer Center,University of Massachusetts Chan Medical School

Journal (Biochemistry)

¹³C₄-Aspartate and ¹³C₅-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

Tenzin Tseyang*¹, Jonathan Valeros*¹, Paula Vo*¹, Jessica B. Spinelli^1,2

¹Program in Molecular Medicine,University of Massachusetts Chan Medical School, ²Cancer Center,University of Massachusetts Chan Medical School

Journal (Biochemistry)

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Alice Sosic¹, Cristina Dal Lago¹, Richard Göttlich², Barbara Gatto¹

¹Department of Pharmaceutical and Pharmacological Sciences,University of Padova, ²Institute of Organic Chemistry,Justus Liebig University Giessen

Journal (Biochemistry)

Folding and Probing of Nucleic Acid Constructs

Alice Sosic¹, Cristina Dal Lago¹, Richard Göttlich², Barbara Gatto¹

¹Department of Pharmaceutical and Pharmacological Sciences,University of Padova, ²Institute of Organic Chemistry,Justus Liebig University Giessen

Journal (Biochemistry)

Separation of Polymerized Nucleic Acid Constructs by PAGE

Alice Sosic¹, Cristina Dal Lago¹, Richard Göttlich², Barbara Gatto¹

¹Department of Pharmaceutical and Pharmacological Sciences,University of Padova, ²Institute of Organic Chemistry,Justus Liebig University Giessen

Journal (Biochemistry)

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Celine Park*¹, Taehyun Yang*¹, Sang-Hyun Rah¹, Hyun Gyu Kim^2,3, Tae-Young Yoon^2,3, Min Ju Shon^1,4

¹Department of Physics,Pohang University of Science and Technology (POSTECH), ²School of Biological Sciences,Seoul National University, ³Institute for Molecular Biology and Genetics,Seoul National University, ⁴School of Interdisciplinary Bioscience and Bioengineering,Pohang University of Science and Technology (POSTECH)

Journal (Biochemistry)

5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

Journal (Biochemistry)

HeLa Cell Fixation with Hoechst 33342 and Blocking

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

Journal (Biochemistry)

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

Journal (Biochemistry)

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

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