1Department of Anatomy and Embryology,Leiden University Medical Center, 2The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW),Leiden University Medical Center, 3Department of Obstetrics and Gynaecology,Amsterdam University Medical Center, 4Centre of Expertise on Gender Dysphoria,Amsterdam UMC, 5Amsterdam Reproduction and Development Research Institute, 6Department of Gynaecology,Leiden University Medical Center, 7Ghent-Fertility and Stem Cell Team (G-FAST), Department of Reproductive Medicine,Ghent University Hospital
Summary
该协议描述了从原代人卵巢表面上皮 （hOSE） 细胞建立三维 （3D） 组织类器官。该方案包括从新鲜收集的卵巢中分离 hOSE、hOSE 的细胞扩增、冷冻保存-解冻程序和类器官衍生。包括免疫荧光、定量分析和作为筛选平台的展示实用程序。
