Elucidating T-Cell Kinetics: Methods for Real-Time Migration Analysis

May 24, 2024

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

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CD8+ T-Cell Purification from Mouse Lymph Nodes and Spleen for Kinetic Studies

02:20 min
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Time-Lapse Imaging to Study Activated Murine CD8+ T-Cell Migration Dynamics

01:02 min
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Tracking Migration of Activated T-Cells Isolated from Mouse Secondary Lymphoid Tissues

02:05 min
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1Department of Microbiology and Immunology,University of Rochester, 2David H. Smith Center for Vaccine Biology and Immunology,University of Rochester

Summary

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This protocol providesa method of primary murine T cell isolation and time-lapse microscopy of T cell migration under specific environmental conditions with quantitative analysis.

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