Real-Time In Vitro Migration Assay for Primary Murine CD8+ T Cells

Allison T. Ryan; Ankit Dahal; Sana Mir; Minsoo Kim; Kihong Lim

doi:10.3791/66580

Journal

免疫与感染

Elucidating T-Cell Kinetics: Methods for Real-Time Migration Analysis

May 24, 2024

Author Spotlight: Understanding Disease Mechanisms Through Real-Time Analysis of T-Cell Migration

01:27 min

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429 Views

CD8+ T-Cell Purification from Mouse Lymph Nodes and Spleen for Kinetic Studies

02:20 min

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95 Views

Time-Lapse Imaging to Study Activated Murine CD8+ T-Cell Migration Dynamics

01:02 min

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52 Views

Tracking Migration of Activated T-Cells Isolated from Mouse Secondary Lymphoid Tissues

02:05 min

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39 Views

Allison T. Ryan^1,2, Ankit Dahal^1,2, Sana Mir^1,2, Minsoo Kim^1,2, Kihong Lim^1,2

¹Department of Microbiology and Immunology,University of Rochester, ²David H. Smith Center for Vaccine Biology and Immunology,University of Rochester

Summary

This protocol providesa method of primary murine T cell isolation and time-lapse microscopy of T cell migration under specific environmental conditions with quantitative analysis.