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Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

January 19th, 2024
*1, *2,3, 1, 2,3, 2,4, 1, 2
1Department Chemistry and Biochemistry, Brigham Young University, 2Eukaryotic Pathogens Innovation Center, Clemson University, 3Department of Genetics and Biochemistry, Clemson University, 4Department of Physics and Astronomy, Clemson University
* These authors contributed equally

Summary
Automatic Translation

We describe a method to study how pH responds to environmental cues in the glycosomes of the bloodstream form of African trypanosomes. This approach involves a pH-sensitive heritable protein sensor in combination with flow cytometry to measure pH dynamics, both as a time-course assay and in a high-throughput screen format.

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