Agrobacterium-Mediated Hairy Root Induction and Regeneration in Plants

December 22, 2023

Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae

01:07 min

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Agrobacterium-Mediated Hairy Root Transformation in Brassica napus DH12075

02:47 min

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Regeneration and Selection Methods for Brassica napus DH12075 Regenerants and T1 Plants

02:11 min

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Hairy Root Transformation and Regeneration in Arabidopsis thaliana Col-0

02:52 min

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Veronika Jedličková, Marie Štefková, Marek Sedláček, Klára Panzarová, Helene S. Robert

¹Hormonal Crosstalk in Plant Development, Mendel Center for Plant Genomics and Proteomics, CEITEC MU-Central European Institute of Technology,Masaryk University, ²PSI – Photon Systems Instruments

Summary

The protocol describes hairy root induction using Arabidopsis primary inflorescence stems and Brassica napus hypocotyls. The hairy roots can be cultured and used as explants to regenerate transgenic plants.