Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility in Drosophila

April 14, 2023

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

02:44 min
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Collection of Drosophila Embryos and Preparing Them for Optogenetic Stimulation

02:03 min
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Optogenetic Stimulation, Laser Ablation, and Imaging of Drosophila Embryos

08:09 min
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1Department of Biological Sciences,Dartmouth College, 2School of Life Sciences,Westlake University, 3Department of Molecular Biology,Princeton University

Summary

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Actomyosin contractility plays an important role in cell and tissue morphogenesis. However, it is challenging to manipulate actomyosin contractility in vivo acutely. This protocol describes an optogenetic system that rapidly inhibits Rho1-mediated actomyosin contractility in Drosophila embryos, revealing the immediate loss of epithelial tension after the inactivation of actomyosin in vivo.

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